Monette F C, Sigounas G
Department of Biology, Boston University, Massachusetts 02215.
Exp Hematol. 1988 Sep;16(8):727-9.
The role of hemin (iron protoporphyrin 9) in the enhancement of interleukin-3 (IL-3)-stimulated multipotent stem cell colony formation was assessed in both serum-containing as well as in "serum-free" marrow culture systems. A greater than 7-fold enhancement in colony number was observed when cultures were supplemented with both IL-3 and hemin compared with either factor alone. In addition, this effect was observed over a wide concentration range. Hemin by itself failed to promote CFU-GEMM in the "serum-free" marrow culture system. The results suggest that hemin acts synergistically with IL-3 to promote the growth of CFU-GEMM in a dose-dependent manner.
在含血清以及“无血清”骨髓培养系统中，评估了氯化血红素（铁原卟啉9）在增强白细胞介素-3（IL-3）刺激的多能干细胞集落形成方面的作用。与单独使用任何一种因子相比，当培养物同时添加IL-3和氯化血红素时，观察到集落数量增加了7倍以上。此外，在很宽的浓度范围内都观察到了这种效应。在“无血清”骨髓培养系统中，氯化血红素本身不能促进CFU-GEMM的形成。结果表明，氯化血红素与IL-3协同作用，以剂量依赖的方式促进CFU-GEMM的生长。
