Tran Quang Hieu, Nguyen Thanh Tan, Pham Kim Phuong
Chemistry Division, Basic Sciences Department, Saigon Technology University, 180 Cao Lo, Ward 4, District 8, Ho Chi Minh City 700000, Vietnam.
Saigon STC Hi-Tech Analytical Center, Ho Chi Minh City 700000, Vietnam.
Int J Anal Chem. 2020 Jun 17;2020:2187646. doi: 10.1155/2020/2187646. eCollection 2020.
A selective, sensitive, and rapid method by using ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for the determination of histamine in fish and fish sauce was developed. The optimal conditions of liquid chromatographic separation and mass spectroscopy of histamine have also been investigated. The linear ranges of the method were 20.0 ÷ 1000 ng/mL, and the corresponding correlation coefficient was 0.9993. Mean recoveries of the analyte at three spike levels (low, medium, and high) were within the range of 98.5% ÷ 102.5% ( = 7). The limit of detection (LOD) and limit of quantification (LOQ) values were 3.83 and 11.50 ng/mL for the fish sauce sample and 4.71 and 14.12 ng/mL for the fish sample, respectively. The influence of the matrix effect on the accuracy, repeatability, and recovery of the method was negligible. The recommended method was applied to determine the content of this substance in 21 fish sauce samples and 4 kinds of fish samples, which were collected from Ho Chi Minh City, Vietnam, in 2019.
建立了一种采用超高效液相色谱-串联质谱法（UPLC-MS/MS）测定鱼类及鱼露中组胺的选择性、灵敏且快速的方法。同时研究了组胺的液相色谱分离和质谱分析的最佳条件。该方法的线性范围为20.0÷1000 ng/mL，相应的相关系数为0.9993。在三个加标水平（低、中、高）下分析物的平均回收率在98.5%÷102.5%范围内（n = 7）。鱼露样品的检测限（LOD）和定量限（LOQ）值分别为3.83和11.50 ng/mL，鱼样品的检测限和定量限分别为4.71和14.12 ng/mL。基质效应对该方法的准确性、重复性和回收率的影响可忽略不计。将所推荐的方法应用于测定2019年从越南胡志明市采集的21份鱼露样品和4种鱼样品中该物质的含量。
