Mehta S, Grant S, Sandler R, Sharma S, Ford R, Maizel A
Department of Molecular Pathology, M.D. Anderson Cancer Center, Houston, TX.
Lymphokine Res. 1988 Summer;7(2):167-73.
A 60 kD cytosolic protein derived from human peripheral blood T cells has been shown to mediate proliferation in BCGF responsive long term B cell cultures. In this report, we show cBCGF-60kD can be radioiodinated with no significant loss of biologic activity and bound specifically to B cell surface membrane receptors. The time course of 125I-cBCGF-60kD demonstrated that binding reached saturation within 40 minutes, (T 1/2 of 15 minutes). Scatchard analyses of ligand-receptor binding identified a high affinity receptor (0.6(-3) x 10(3) receptors per cell) having a dissociation constant of 1-8 pM. Partial cross-competition experiments with a secreted, T cell derived BCGF-12kD identified a potential receptor heterogeneity on BCGF responsive B cell line.
一种源自人外周血T细胞的60kD胞质蛋白已被证明可介导卡介苗生长因子（BCGF）反应性长期B细胞培养中的增殖。在本报告中，我们表明cBCGF - 60kD可以进行放射性碘化，而生物活性没有显著损失，并且能特异性结合到B细胞表面膜受体上。125I - cBCGF - 60kD的时间进程表明，结合在40分钟内达到饱和（半衰期为15分钟）。配体 - 受体结合的Scatchard分析确定了一种高亲和力受体（每个细胞0.6×10³个受体），其解离常数为1 - 8pM。用一种分泌型、T细胞衍生的BCGF - 12kD进行的部分交叉竞争实验确定了BCGF反应性B细胞系上存在潜在的受体异质性。
