正常人皮肤来源的淋巴细胞中白细胞介素-1的鉴定。
Identification of interleukin-1 in normal human lymph derived from skin.
作者信息
Plachta J, Olszewski W L, Grzelak I, Engeset A, Cholewka W
机构信息
Department of Surgical Research & Transplantology, Polish Academy of Sciences, Warsaw.
出版信息
Lymphokine Res. 1988 Summer;7(2):93-7.
Interleukin 1 was isolated from human lymph drained from normal skin. The lymph was separated by gel filtration chromatography on Spherogel TSK SW 2000 column. IL-1 activity was found in three fractions: greater than 70 kD, 13-16 kD and 5-5.5 kD. Additionally a slight IL-1 activity was found in the 36-40 kD fraction. When exogenous IL-1 was added to the lymph inhibitory activity was detected in fractions: greater than 70 kD, 22-30 kD and 5.5-8 kD.
白细胞介素1是从正常皮肤引流的人体淋巴液中分离出来的。淋巴液通过在Spherogel TSK SW 2000柱上进行凝胶过滤色谱法进行分离。在三个组分中发现了IL-1活性：大于70 kD、13 - 16 kD和5 - 5.5 kD。此外，在36 - 40 kD组分中发现了轻微的IL-1活性。当向淋巴液中添加外源性IL-1时，在大于70 kD、22 - 30 kD和5.5 - 8 kD的组分中检测到抑制活性。
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