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微流控免疫分析用于在 15 分钟内灵敏且同时检测 SARS-CoV-2 的 IgG/IgM/抗原

Microfluidic Immunoassays for Sensitive and Simultaneous Detection of IgG/IgM/Antigen of SARS-CoV-2 within 15 min.

机构信息

Shanghai Suxin Biotechnology Co. Ltd., Shanghai 201318, P. R. China.

NingBo iGeneTec Technology Co. Ltd., Ningbo 315000, P. R. China.

出版信息

Anal Chem. 2020 Jul 21;92(14):9454-9458. doi: 10.1021/acs.analchem.0c01635. Epub 2020 Jul 9.

DOI:10.1021/acs.analchem.0c01635
PMID:32615038
Abstract

The outbreak of SARS-CoV-2 is posing serious global public health problems. Facing the emergence of this pandemic, we established a portable microfluidic immunoassay system for easy-to-use, sensitive, rapid (<15 min), multiple, and on-site detection of IgG/IgM/Antigen of SARS-CoV-2 simultaneously. This integrated method was successfully applied for detecting SARS-CoV-2 IgM and IgG antibodies in clinical human serum as well as SARS-CoV-2 antigen in pharyngeal swabs from 26 patients with COVID-19 infection and 28 uninfected people. The assay demonstrated high sensitivity and specificity, which is promising for the diagnosis and monitoring as well as control of SARS-CoV-2 worldwide.

摘要

SARS-CoV-2 的爆发给全球公共卫生带来了严重的问题。面对这一流行病的出现，我们建立了一种便携式微流控免疫分析系统，用于方便、敏感、快速（<15 分钟）、多通道和现场同时检测 SARS-CoV-2 的 IgG/IgM/抗原。该集成方法成功应用于检测临床人血清中的 SARS-CoV-2 IgM 和 IgG 抗体，以及 26 例 COVID-19 感染患者和 28 名未感染者的咽拭子中的 SARS-CoV-2 抗原。该检测方法具有较高的灵敏度和特异性，有望用于 SARS-CoV-2 的诊断、监测和全球控制。

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