Department of Gastroenterological and Transplant Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan; Research Center for Hepatology and Gastroenterology, Hiroshima University, Hiroshima, Japan.
Department of Gastroenterological and Transplant Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan; Research Center for Hepatology and Gastroenterology, Hiroshima University, Hiroshima, Japan.
Int J Surg. 2020 Aug;80:84-90. doi: 10.1016/j.ijsu.2020.05.007. Epub 2020 Jun 29.
This study examined whether single nucleotide polymorphism (SNP) in programmed cell death protein (PD)-1 is related to the postoperative prognosis of patients with hepatocellular carcinoma (HCC). The immune checkpoint protein PD-1 is an important inhibitor of T cell responses. SNP in the promoter region of PD-1 -606 G/A has been reported to result in high activation and expression of PD-1 associated with cancer risk.
We analyzed 321 patients with HCC who underwent hepatectomy between 2010 and 2015. PD-1 SNP was analyzed by polymerase chain reaction, and the prognosis after surgical treatment of patients with HCC was analyzed.
The PD-1 SNP statuses were as follows: 90 AA (28.1%), 163 GA (50.8%), 68 GG (21.2%). The baseline parameters did not statistically differ between the three groups. The overall survival (OS) of patients with the GG genotype was significantly lower than that of those with the other genotypes (P = 0.031). The GG genotype was an independent risk factor for OS (P = 0.009; HR 2.201). There was no significant difference between the GG genotype and other genotypes in recurrent-free survival. The extrahepatic recurrence (EHR) rate of those with the GG genotype was significantly higher than that of those with the other genotypes (P = 0.036). The GG genotype was an independent risk factor for EHR (P = 0.008; HR 2.037).
The PD-1 SNP GG genotype is associated with poor survival and increased EHR in HCC. Furthermore, the GG genotype is an independent predictive factor for OS and EHR.
本研究旨在探讨细胞程序性死亡蛋白 1（PD-1）的单核苷酸多态性（SNP）是否与肝细胞癌（HCC）患者的术后预后相关。免疫检查点蛋白 PD-1 是 T 细胞反应的重要抑制剂。已有报道称，PD-1 启动子区域的 SNP-606G/A 导致 PD-1 高度激活和表达，与癌症风险相关。
我们分析了 2010 年至 2015 年间接受肝切除术的 321 例 HCC 患者。通过聚合酶链反应分析 PD-1 SNP，分析 HCC 患者手术后的预后。
PD-1 SNP 状态如下：90 AA（28.1%）、163 GA（50.8%）、68 GG（21.2%）。三组间基线参数无统计学差异。GG 基因型患者的总生存期（OS）明显低于其他基因型（P=0.031）。GG 基因型是 OS 的独立危险因素（P=0.009；HR 2.201）。GG 基因型与其他基因型在无复发生存方面无显著差异。GG 基因型患者的肝外复发（EHR）率明显高于其他基因型（P=0.036）。GG 基因型是 EHR 的独立危险因素（P=0.008；HR 2.037）。
PD-1 SNP GG 基因型与 HCC 患者的生存不良和 EHR 增加相关。此外，GG 基因型是 OS 和 EHR 的独立预测因子。
