Guo Jiaqing, Ye Shuai, Li Hao, Song Jun, Qu Junle
Center for Biomedical Optics and Photonics (CBOP) & College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems, Shenzhen University, Shenzhen 518060, PR China.
Center for Biomedical Optics and Photonics (CBOP) & College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems, Shenzhen University, Shenzhen 518060, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Oct 15;240:118592. doi: 10.1016/j.saa.2020.118592. Epub 2020 Jun 13.
Low-toxic and environmentally friendly carbon dots (CDs) have been extensively applied in various fields. CDs usually demonstrate excellent selectivity and high sensitivity, especially in ion detection. However, the most commonly used CDs are excited by ultraviolet (UV) light and emit weak fluorescence light, limiting their application in some fields. Herein, novel fluorine and nitrogen codoped carbon dots (FNCDs) were prepared by a simple hydrothermal method and used as a fluorescent probe for ion detection. The FNCDs were excited by blue light and emitted strong green fluorescence, and the photoluminescence quantum yield was as high as 56.7%. The fluorescence of the FNCDs could be rapidly quenched by ClO ions, indicating their potential application for ClO detection. The fluorescence of the FNCDs was quenched by ClO ions in less than 1 min, and the intensity of the fluorescence decreased linearly as the ClO concentration increased from 0 to 20 μM. The detection limit was calculated to be as low as 8.2 nM, indicating high sensitivity of the FNCDs probe. The quench effect of the ClO ions on the FNCDs probe fluorescence was not affected by other ions, demonstrating excellent selectivity of the FNCDs probe. Because of their excellent biological compatibility, the FNCDs were also successfully used to identify exogenous ClO in living cells. These FNCDs have promising prospects as novel sensitive and inexpensive probes for the detection of pollutants and in the pathological studies of clinical diseases.
低毒且环保的碳点（CDs）已在各个领域得到广泛应用。碳点通常表现出优异的选择性和高灵敏度，尤其是在离子检测方面。然而，最常用的碳点由紫外（UV）光激发并发出微弱荧光，这限制了它们在某些领域的应用。在此，通过简单的水热法制备了新型氟氮共掺杂碳点（FNCDs），并将其用作离子检测的荧光探针。FNCDs由蓝光激发并发出强烈的绿色荧光，其光致发光量子产率高达56.7%。FNCDs的荧光可被ClO离子快速猝灭，表明它们在ClO检测方面具有潜在应用。FNCDs的荧光在不到1分钟内被ClO离子猝灭，并且随着ClO浓度从0增加到20μM，荧光强度呈线性下降。计算得出检测限低至8.2 nM，表明FNCDs探针具有高灵敏度。ClO离子对FNCDs探针荧光的猝灭效应不受其他离子影响，证明了FNCDs探针具有优异的选择性。由于其优异的生物相容性，FNCDs还成功用于识别活细胞中的外源性ClO。这些FNCDs作为检测污染物的新型灵敏且廉价的探针以及在临床疾病的病理研究中具有广阔的前景。
