Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agriculture Sciences, Beijing 100081, China.
Bioresour Technol. 2020 Oct;313:123702. doi: 10.1016/j.biortech.2020.123702. Epub 2020 Jun 17.
Aiming at relieving acid accumulation in anaerobic digestion (AD), syntrophic associations of syntrophic fatty acid-oxidation bacteria and H/formate-scavenging methanogens were enriched by feeding propionate, butyrate and formate in an up-flow anaerobic sludge blanket (UASB) reactor. Results showed that methane yield increased by 50% with increasing formate concentration (0-2000 mg COD/L). In addition, the abundance and quantity of SFOB (Syntrophobacter, Smithella and Syntrophomonas) and H/formate-scavenging methanogens (Methanobacteriales and Methanomicrobiales) were increased after microbial acclimation. The enriched syntrophic associations showed higher propionate and butyrate removal efficiencies of 98.48 ± 1.14% and 99.71 ± 0.71%, respectively. Furthermore, encoding genes of formate dehydrogenase and hydrogenases presented higher abundances after microbial enrichment, which suggested that the enhancements of interspecies formate transfer and interspecies hydrogen transfer between syntrophic associations benefited volatile fatty acids (VFAs) conversion. This research provided an effective strategy to relieve acid accumulation.
针对缓解厌氧消化（AD）中的酸积累问题，通过在升流式厌氧污泥床（UASB）反应器中添加丙酸、丁酸和甲酸盐，富集了产酸脂肪酸氧化菌和 H/甲酸盐清除产甲烷菌的共培养物。结果表明，随着甲酸盐浓度（0-2000mg COD/L）的增加，甲烷产量增加了 50%。此外，在微生物驯化后，SFOB（Syntrophobacter、Smithella 和 Syntrophomonas）和 H/甲酸盐清除产甲烷菌（Methanobacteriales 和 Methanomicrobiales）的丰度和数量增加。富集的共培养物对丙酸和丁酸的去除效率分别达到 98.48±1.14%和 99.71±0.71%。此外，微生物富集后甲酸脱氢酶和氢化酶的编码基因丰度更高，这表明共培养物之间种间甲酸转移和种间氢转移的增强有利于挥发性脂肪酸（VFAs）的转化。本研究提供了一种有效缓解酸积累的策略。
