长链非编码 RNA POU3F3 通过下调 microRNA-30d-5p 促进非小细胞肺癌（腺癌）中癌细胞的增殖、迁移和侵袭。
Long noncoding RNA POU3F3 enhances cancer cell proliferation, migration and invasion in non-small cell lung cancer (adenocarcinoma) by downregulating microRNA-30d-5p.
机构信息
Department of Respiratory Medicine, Guangdong Provincial Hospital of Integrated Traditional Chinese and Western Medicine, No. 16 Nanwu Road, Guicheng, Nanhai District, Foshan City, Guangdong Province, 528200, P.R. China.
Clinic of Integrated Traditional & Western Medicine, Shenzhen Longgang District People's Hospital, Shenzhen City, 518172, P.R. China.
出版信息
BMC Pulm Med. 2020 Jul 2;20(1):185. doi: 10.1186/s12890-020-01218-3.
BACKGROUND
Long noncoding RNA POU class 3 homeobox 3 (POU3F3) is upregulated in esophageal squamous-cell carcinomas. The present study aimed to investigate the role of POU3F3 in non-small cell lung cancer (NSCLC).
METHODS
A total of 80 patients with NSCLC (adenocarcinoma) admitted by Guangdong Provincial Hospital of Integrated Traditional Chinese and Western Medicine between May 2016 and May 2018 were enrolled in this study. All patients were diagnosed by histopathological approaches. Expression levels of POU3F3 and microRNA-30d-5p (miR-30d-5p) in cancer and non-tumor tissues from these NSCLC patients were determined by qRT-PCR. Cell transfections were performed to assess interactions between miR-30d-5p and POU3F3. Cell proliferation, Transwell migration and invasion assays were performed to investigate the role of miR-30d-5p and POU3F3 in the regulation of cell proliferation, migration and invasion.
RESULTS
POU3F3 was upregulated, while miR-30d-5p was downregulated in cancer tissues than in adjacent healthy tissues of NSCLC patients. Correlation analysis showed that expression levels of POU3F3 and miR-30d-5p were inversely correlated in tumor tissues. Overexpression of miR-30d-5p did not affect the expression of POU3F3, while overexpression of POU3F3 resulted in the suppression of miR-30d-5p in NSCLC cell lines. Overexpression of POU3F3 mediated enhanced proliferation, migration and invasion of NSCLC cells. In addition, overexpression of miR-30d-5p played an opposite role and attenuated the effects of overexpressing POU3F3 on cancer cell proliferation, migration and invasion.
CONCLUSIONS
POU3F3 might positively regulate NSCLC cell proliferation, migration and invasion through downregulation of miR-30d-5p.
背景
长链非编码 RNA POU 类 3 同源框 3（POU3F3）在食管鳞状细胞癌中上调。本研究旨在探讨 POU3F3 在非小细胞肺癌（NSCLC）中的作用。
方法
本研究纳入 2016 年 5 月至 2018 年 5 月期间广东药科大学附属第一医院收治的 80 例（腺癌）NSCLC 患者。所有患者均经组织病理学方法诊断。采用 qRT-PCR 检测这些 NSCLC 患者癌组织和非癌组织中 POU3F3 和 microRNA-30d-5p（miR-30d-5p）的表达水平。通过细胞转染评估 miR-30d-5p 与 POU3F3 之间的相互作用。通过细胞增殖、Transwell 迁移和侵袭实验研究 miR-30d-5p 和 POU3F3 在调节细胞增殖、迁移和侵袭中的作用。
结果
与 NSCLC 患者癌旁正常组织相比，癌组织中 POU3F3 上调，miR-30d-5p 下调。相关性分析显示，肿瘤组织中 POU3F3 和 miR-30d-5p 的表达水平呈负相关。过表达 miR-30d-5p 不影响 POU3F3 的表达，而过表达 POU3F3 导致 NSCLC 细胞系中 miR-30d-5p 的抑制。过表达 POU3F3 介导 NSCLC 细胞增殖、迁移和侵袭增强。此外，过表达 miR-30d-5p 发挥相反作用，减弱了过表达 POU3F3 对癌细胞增殖、迁移和侵袭的影响。
结论
POU3F3 可能通过下调 miR-30d-5p 正向调节 NSCLC 细胞的增殖、迁移和侵袭。
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