Xu Yan, Wang Yanbo, Guo Yinan, Wei Lina, Ding Lizhong, Wang Zhongtian, Sun Liping
Changchun University of Chinese Medicine, Changchun, Jilin 130117, China.
Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, Jilin 130021, China.
Evid Based Complement Alternat Med. 2020 Jun 16;2020:8537026. doi: 10.1155/2020/8537026. eCollection 2020.
Pore-forming toxins produced by bacteria are some of the most important molecular weapons for bacterial virulence. Pneumolysin (PLY) is a pore-forming toxin secreted by () and plays a vital role in the spread, colonization, and invasion of this bacterium in the host, indicating that PLY is a promising target for developing treatments against infection. In this study, Cortex granules (CCCGs), a prescription drug on the market, were shown to inhibit the pore-forming activity of PLY and protect against PLY-mediated cell hemolysis and A549 cell death without antibacterial activity or inhibition of PLY production. In addition, CCCG treatment inhibited the oligomerization of PLY. Animal experiments showed that CCCGs can reduce the death of mice infected with , the degree of pathological damage to the lungs, and the levels of TNF- and IL-6 in the lungs. In summary, our results demonstrated that CCCGs, a marketed Chinese medicine, inhibit PLY activity and subsequently attenuate virulence, which would offer a novel strategy for fighting infection and a new use for CCCGs.
细菌产生的成孔毒素是细菌毒力最重要的分子武器之一。肺炎溶血素（PLY）是由（）分泌的一种成孔毒素，在该细菌在宿主体内的传播、定植和侵袭中起着至关重要的作用，这表明PLY是开发针对（）感染治疗方法的一个有前景的靶点。在本研究中，市售处方药复方陈香胃片（CCCGs）被证明可抑制PLY的成孔活性，并防止PLY介导的细胞溶血和A549细胞死亡，且无抗菌活性或抑制PLY产生。此外，CCCGs处理可抑制PLY的寡聚化。动物实验表明，CCCGs可降低感染（）的小鼠的死亡率、肺部病理损伤程度以及肺中TNF-和IL-6的水平。总之，我们的结果表明，市售中药CCCGs可抑制PLY活性，进而减弱（）的毒力，这将为对抗（）感染提供一种新策略，并为CCCGs提供一种新用途。
