从韦氏芽孢杆菌中鉴定出一种 L-阿拉伯糖异构酶及其在 L-核酮糖和 L-核糖生物合成中的应用。
Characterization of an L-Arabinose Isomerase from Bacillus velezensis and Its Application for L-Ribulose and L-Ribose Biosynthesis.
机构信息
The Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China.
Jiangsu Key Lab for the Chemistry & Utilization of Agricultural and Forest Biomass, Nanjing Forestry University, Nanjing, 210037, China.
出版信息
Appl Biochem Biotechnol. 2020 Nov;192(3):935-951. doi: 10.1007/s12010-020-03380-0. Epub 2020 Jul 2.
L-Ribulose and L-ribose are two high-value unnatural sugars that can be biosynthesized by sugar isomerases. In this paper, an L-arabinose isomerase (BvAI) from Bacillus velezensis CICC 24777 was cloned and overexpressed in Escherichia coli BL21 (DE3) strain. The maximum activity of recombinant BvAI was observed at 45 °C and pH 8.0, in the presence of 1.0 mM Mn. Approximately 207.2 g/L L-ribulose was obtained from 300 g/L L-arabinose in 1.5 h by E. coli harboring BvAI. In addition, approximately 74.25 g/L L-ribose was produced from 300 g/L L-arabinose in 7 h by E. coli co-expressing BvAI and L-RI from Actinotalea fermentans ATCC 43279 (AfRI). This study provides a feasible approach for producing L-ribose from L-arabinose using a co-expression system harboring L-Al and L-RI.
L-核酮糖和 L-核糖是两种高附加值的非天然糖，可以通过糖异构酶生物合成。在本文中，我们从解淀粉芽孢杆菌 BvAI 中克隆并在大肠杆菌 BL21 (DE3) 菌株中过表达了一种 L-阿拉伯糖异构酶（BvAI）。重组 BvAI 的最大活性在 45°C 和 pH 8.0 下，在 1.0 mM Mn 的存在下观察到。大肠杆菌中含有 BvAI 时，可在 1.5 小时内从 300g/L 的 L-阿拉伯糖中获得约 207.2 g/L 的 L-核酮糖。此外，在大肠杆菌中同时表达 BvAI 和来自发酵棒杆菌 ATCC 43279 的 L-RI（AfRI）时，可在 7 小时内从 300 g/L 的 L-阿拉伯糖中生产约 74.25 g/L 的 L-核糖。该研究为使用同时表达 L-Al 和 L-RI 的共表达系统从 L-阿拉伯糖生产 L-核糖提供了一种可行的方法。
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