反向遗传学方法在布尼亚病毒疫苗研发中的应用。
Reverse genetics approaches for the development of bunyavirus vaccines.
机构信息
Departments of Microbiology and Immunology and, the University of Texas Medical Branch, Galveston, TX 77555-1019, United States.
Departments of Microbiology and Immunology and, the University of Texas Medical Branch, Galveston, TX 77555-1019, United States; Institute of Human Infection and Immunity and, the University of Texas Medical Branch, Galveston, TX 77555-1019, United States; Center for Biodefense and Emerging Infectious Diseases and, the University of Texas Medical Branch, Galveston, TX 77555-1019, United States; UTMB Center for Tropical Diseases and, the University of Texas Medical Branch, Galveston, TX 77555-1019, United States; The Sealy Institute for Vaccine Sciences and, the University of Texas Medical Branch, Galveston, TX 77555-1019, United States.
出版信息
Curr Opin Virol. 2020 Oct;44:16-25. doi: 10.1016/j.coviro.2020.05.004. Epub 2020 Jun 30.
The Bunyavirales order is the largest group of RNA viruses, which includes important human and animal pathogens, that cause serious diseases. Licensed vaccines are often not available for many of these pathogens. The establishment of bunyavirus reverse genetics systems has facilitated the generation of recombinant infectious viruses, which have been employed as powerful tools for understanding bunyavirus biology and identifying important virulence factors. Technological advances in this area have enabled the development of novel strategies, including codon-deoptimization, viral genome rearrangement and single-cycle replicable viruses, for the generation of live-attenuated vaccine candidates. In this review, we have summarized the current knowledge of the bunyavirus reverse genetics approaches for the generation of live-attenuated vaccine candidates and their evaluation in animal models.
布尼亚病毒目是最大的 RNA 病毒群，其中包括重要的人类和动物病原体，这些病原体可导致严重疾病。对于许多这些病原体，通常没有许可的疫苗。布尼亚病毒反向遗传学系统的建立促进了重组传染性病毒的产生，这些病毒已被用作了解布尼亚病毒生物学和鉴定重要毒力因子的有力工具。该领域的技术进步使开发新策略成为可能，包括密码子去优化、病毒基因组重排和单周期复制病毒，以产生减毒活疫苗候选物。在这篇综述中，我们总结了布尼亚病毒反向遗传学方法在产生减毒活疫苗候选物及其在动物模型中的评估方面的最新知识。
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