Iakubov L Z, Sakharova A V, Romanova N V, Cherepakhin V V, Rokhlin O V
Biull Eksp Biol Med. 1988 Aug;106(8):200-2.
Neonatal rat hybridomas were tested for natural autoantibodies (NAA) production, using different screening procedures. NAA were discovered in 35% of immunoglobulins producing hybridomas. Radioimmunoassay (RIA) on brain and liver homogenates and immunocytochemistry on brain sections are the procedures of choice revealing the major part of the identified NAA. On the contrary, only a small portion of NAA could be detected with indirect immunofluorescence on fixed fibroblasts and with RIA on individual autoantigens. All the NAA revealed proved to be of the IgM type and almost all of them possessed neither organ nor species-specificity. In spite of that, most of the NAA reacted with definite cell populations of nervous tissue such as glia, neurons, ependyma or brain vessel cells. The studied panel of NAA from neonatal rats has common features with similar panels from newborn and old mice, though some species-specific characteristics do exist.
使用不同的筛选程序对新生大鼠杂交瘤进行天然自身抗体（NAA）产生的测试。在35%产生免疫球蛋白的杂交瘤中发现了NAA。对脑和肝匀浆进行放射免疫测定（RIA）以及对脑切片进行免疫细胞化学是揭示已鉴定NAA主要部分的首选方法。相反，通过对固定成纤维细胞进行间接免疫荧光以及对单个自身抗原进行RIA，只能检测到一小部分NAA。所有检测到的NAA均为IgM型，并且几乎所有NAA都不具有器官或物种特异性。尽管如此，大多数NAA与神经组织的特定细胞群体发生反应，如神经胶质细胞、神经元、室管膜细胞或脑血管细胞。来自新生大鼠的NAA研究组与来自新生和老年小鼠的类似研究组具有共同特征，不过确实存在一些物种特异性特征。
