United States Department of Agriculture -Agricultural Research Service, San Joaquin Valley Agricultural Sciences Center, Parlier, CA, 93648, USA.
Department of Plant Pathology, University of California, Davis, CA, 95616, USA.
BMC Res Notes. 2020 Jul 3;13(1):320. doi: 10.1186/s13104-020-05160-9.
Spiroplasma citri is a bacterium with a wide host range and is the causal agent of citrus stubborn and brittle root diseases of citrus and horseradish, respectively. S. citri is transmitted in a circulative, persistent manner by the beet leafhopper, Neoaliturus (Circulifer) tenellus (Baker), in North America. Five strains of S. citri were cultured from citrus, horseradish, and N. tenellus from different habitats and times. DNA from cultures were sequenced and genome assembled to expand the database to improve detection assays and better understand its genetics and evolution.
The whole genome sequence of five strains of S. citri are described herein. The S. citri chromosome was circularized for all five strains and ranged from 1,576,550 to 1,742,208 bp with a G + C content of 25.4-25.6%. Characterization of extrachromosomal DNAs resulted in identification of one or two plasmids, with a G + C content of 23.3 to 27.6%, from plant hosts; and eight or nine plasmids, with a G + C content of 21.65 to 29.19%, from N. tenellus. Total genome size ranged from 1,611,714 to 1,832,173 bp from plants and 1,968,976 to 2,155,613 bp from the leafhopper. All sequence data has been deposited in DDBJ/ENA/GenBank under the accession numbers CP046368-CP046373 and CP047426-CP047446.
柑橘僵化螺原体是一种宿主范围广泛的细菌，分别是柑橘和辣根柑橘顽固和脆弱根病的病原体。柑橘僵化螺原体由北美叶蝉，Neosaliturus (Circulifer) tenellus (Baker) 以循环、持续的方式传播。从不同栖息地和时间的柑橘、辣根和 N. tenellus 中培养了 5 株 S. citri。从培养物中提取 DNA 进行测序和基因组组装，以扩展数据库，改进检测方法，并更好地了解其遗传和进化。
本文描述了 5 株 S. citri 的全基因组序列。所有 5 株 S. citri 的染色体均已环状化，范围从 1,576,550 到 1,742,208 bp，G+C 含量为 25.4-25.6%。对染色体外 DNA 的特征分析导致鉴定出一个或两个质粒，其 G+C 含量为 23.3 至 27.6%，来自植物宿主；来自 N. tenellus 的八个或九个质粒，G+C 含量为 21.65 至 29.19%。从植物中获得的总基因组大小范围为 1,611,714 至 1,832,173 bp，从叶蝉中获得的总基因组大小范围为 1,968,976 至 2,155,613 bp。所有序列数据已在 DDBJ/ENA/GenBank 中以 CP046368-CP046373 和 CP047426-CP047446 的登录号进行了存储。
