Wu B Q, Fang W G, Zheng J, Sun Y K, Wang J L, Zhao X S, Yang J S, Lu C P, Sun J, Xu Q M
Beijing Medical University.
Zhonghua Zhong Liu Za Zhi. 1988 Jan;10(1):1-3.
Beige-nude mice with combined T and NK cell deficiency were produced by introducing "nu" gene into the beige mice of C57BL/6 background. The beige-nude mice were characterized by morphological features, level and activity of the NK cell in spleen and peripheral blood. NK cell level of the beige-nude mice was higher than the beige mice and similar to the ordinary nude mice though its NK cell activity was markedly lower than the nude mice. Human lung adenocarcinoma (PAa) was transplanted subcutaneously to the beige-nude mice and nude mice of BALB/cA background. No metastasis was found in BALB/cA nude mice (0/7), while in beige-nude mice, spontaneous lymph node metastasis rate was 36.3% (4/11). It should be noted that the transplanted PAa metastasized to lungs, which had never been seen in BALB/cA nude mice in previous experiments. The increase of metastatic rate is correlated with the relative low NK cell activity of the beige-nude mice. Our observation is that the beige-nude mice could be a suitable experimental model for in vivo study on metastatic behavior of the human tumors.
通过将“nu”基因导入C57BL/6背景的米色小鼠中，培育出T细胞和NK细胞联合缺陷的米色裸鼠。对米色裸鼠的形态特征、脾脏和外周血中NK细胞的水平及活性进行了表征。米色裸鼠的NK细胞水平高于米色小鼠，与普通裸鼠相似，但其NK细胞活性明显低于裸鼠。将人肺腺癌（PAa）皮下移植到BALB/cA背景的米色裸鼠和裸鼠体内。在BALB/cA裸鼠中未发现转移（0/7），而在米色裸鼠中，自发淋巴结转移率为36.3%（4/11）。需要注意的是，移植的PAa转移到了肺部，这在之前的实验中从未在BALB/cA裸鼠中见过。转移率的增加与米色裸鼠相对较低的NK细胞活性相关。我们的观察结果是，米色裸鼠可能是用于人类肿瘤转移行为体内研究的合适实验模型。
