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基于胞嘧啶和鸟嘌呤丰富序列的荧光寡核苷酸探针的研制。

Development of fluorescence oligonucleotide probes based on cytosine- and guanine-rich sequences.

机构信息

Faculty of Chemistry, Adam Mickiewicz University, Uniwersytetu Poznanskiego 8, 61-614, Poznan, Poland.

Institute of Bioorganic Chemistry, Polish Academy of Science, Noskowskiego 12/14, 60-704, Poznan, Poland.

出版信息

Sci Rep. 2020 Jul 3;10(1):11006. doi: 10.1038/s41598-020-67745-5.

DOI:10.1038/s41598-020-67745-5
PMID:32620895
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7335195/
Abstract

The properties of cytosine- and guanine-rich oligonucleotides contributed to employing them as sensing elements in various biosensors. In this paper, we report our current development of fluorescence oligonucleotide probes based on i-motif or G-quadruplex forming oligonucleotides for cellular measurements or bioimaging applications. Additionally, we also focus on the spectral properties of the new fluorescent silver nanoclusters based system (ChONC12-AgNCs) that is able to anchor at the Langmuir monolayer interface, which is mimicking the surface of living cells membrane.

摘要

富含胞嘧啶和鸟嘌呤的寡核苷酸的特性有助于将其用作各种生物传感器中的传感元件。在本文中，我们报告了基于 i-motif 或 G-四链体形成寡核苷酸的荧光寡核苷酸探针在细胞测量或生物成像应用方面的最新进展。此外，我们还专注于新型基于荧光银纳米簇的体系（ChONC12-AgNCs）的光谱特性，该体系能够锚定在模拟活细胞膜表面的朗缪尔单层界面上。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/e1de6a1270c2/41598_2020_67745_Sch2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/ff00953f9ee8/41598_2020_67745_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/6614b5320386/41598_2020_67745_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/6f8f600208dc/41598_2020_67745_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/647edebfa5fa/41598_2020_67745_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/37fd1b4d27a8/41598_2020_67745_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/b4a344b2c59b/41598_2020_67745_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/8fffd41fe72d/41598_2020_67745_Sch1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/c14846a02f02/41598_2020_67745_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/7ecc5cda9ebb/41598_2020_67745_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/74b7071a40a3/41598_2020_67745_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/e1de6a1270c2/41598_2020_67745_Sch2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/ff00953f9ee8/41598_2020_67745_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/6614b5320386/41598_2020_67745_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/6f8f600208dc/41598_2020_67745_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/647edebfa5fa/41598_2020_67745_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/37fd1b4d27a8/41598_2020_67745_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/b4a344b2c59b/41598_2020_67745_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/8fffd41fe72d/41598_2020_67745_Sch1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/c14846a02f02/41598_2020_67745_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/7ecc5cda9ebb/41598_2020_67745_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/74b7071a40a3/41598_2020_67745_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/523a/7335195/e1de6a1270c2/41598_2020_67745_Sch2_HTML.jpg

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