Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Beijing Center for Disease Control and Prevention, Beijing 10013, China.
J Antimicrob Chemother. 2020 Oct 1;75(10):2769-2772. doi: 10.1093/jac/dkaa260.
This study was designed to understand the prevalence of antibiotic-resistant bacteria in the Beijing subway environment and the potential transmission of carbapenem-resistant Enterobacteriaceae in a public transportation environment.
Carbapenem-resistant isolates were selected on brain heart infusion agar supplemented with meropenem (0.5 mg/L) and antimicrobial susceptibility testing was conducted using the broth microdilution method. WGS analyses were conducted for 11 Klebsiella pneumoniae isolates to identify resistance genes. The genetic relationships among the isolates were evaluated by MLST and PFGE.
We identified 11 carbapenem-resistant K. pneumoniae isolates from the Beijing subway environment. WGS revealed three STs among the 11 isolates, with 9 isolates classified as ST726 and containing a blaNDM-5-carrying IncX3 plasmid. The genetic environment of blaNDM-5 was very similar to that observed in other blaNDM-5-containing clinical isolates.
The presence of carbapenem-resistant Enterobacteriaceae in a public transportation environment is concerning and indicates that regular antimicrobial resistance surveillance is urgent and necessary.
本研究旨在了解北京地铁环境中抗生素耐药菌的流行情况，以及在公共交通环境中碳青霉烯类耐药肠杆菌科细菌的潜在传播情况。
在含有美罗培南（0.5mg/L）的脑心浸液琼脂上选择碳青霉烯类耐药分离株，并采用肉汤微量稀释法进行抗菌药物敏感性试验。对 11 株肺炎克雷伯菌分离株进行 WGS 分析以鉴定耐药基因。通过 MLST 和 PFGE 评估分离株之间的遗传关系。
我们从北京地铁环境中鉴定出 11 株碳青霉烯类耐药肺炎克雷伯菌。WGS 显示 11 株分离株中有 3 种 ST，其中 9 株被归类为 ST726 ，并含有携带 blaNDM-5 的 IncX3 质粒。blaNDM-5 的遗传环境与其他携带 blaNDM-5 的临床分离株非常相似。
公共交通环境中存在碳青霉烯类耐药肠杆菌科细菌令人担忧，表明迫切需要定期进行抗菌药物耐药性监测。
