Department of Medical Technology, Faculty of Health Science, Kumamoto Health Science University, Kumamoto, Japan.
Department of Cardiovascular Surgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan.
J Artif Organs. 2021 Mar;24(1):22-26. doi: 10.1007/s10047-020-01192-x. Epub 2020 Jul 4.
Roller pumping results in hemolysis and adverse effects on coagulation, but there are few reports on the influence of roller heads on platelets. Here, we evaluate the interaction between roller pumping and platelet function using a simulated extracorporeal circuit incorporating a vinyl chloride tube and roller head pump with 30 min recirculation. Platelet aggregation, platelet count, microparticle, P-selectin, Phosphatidylserine (PS) exposure and Ricinus Communis Agglutinin 1 (RCA-1) were measured before, 5, 10, 20, and 30 min after the recirculation using 100 ml of fresh human blood that had obtained from healthy volunteers (n = 9). Platelet aggregation and platelet count gradually decreased but microparticles significantly increased after the recirculation (P < 0.05). P-selectin, PS exposure and RCA-1 were measured using flow cytometry. There were no significant differences in the P-selectin and PS exposure expression during recirculation. RCA-1, a platelet apoptosis markers, significantly increased 30 min after recirculation (P < 0.05). We thus conclude that roller pumping induced platelet apoptosis and caused decreases in platelet count and aggregation after the recirculation.
滚压泵导致溶血和凝血功能不良，但关于滚轮头对血小板影响的报道较少。本研究采用含氯乙烯管和滚压头泵的模拟体外循环回路，进行 30 分钟再循环，评估滚压泵与血小板功能之间的相互作用。用 100ml 取自健康志愿者的新鲜人血（n=9），在再循环前、再循环后 5、10、20 和 30 分钟，测量血小板聚集、血小板计数、微颗粒、P 选择素、磷脂酰丝氨酸（PS）暴露和蓖麻凝集素 1（RCA-1）。再循环后血小板聚集和血小板计数逐渐下降，但微颗粒显著增加（P<0.05）。用流式细胞术测量 P 选择素和 PS 暴露。再循环过程中 P 选择素和 PS 暴露表达无显著差异。血小板凋亡标志物 RCA-1 在再循环 30 分钟后显著增加（P<0.05）。因此，我们得出结论，滚压泵诱导血小板凋亡，并在再循环后导致血小板计数和聚集减少。
