Microbiota-Host Interactions and Clostridia Research Group, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago, Chile.
Microbiota-Host Interactions and Clostridia Research Group, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago, Chile; Millennium Nucleus in the Biology of Intestinal Microbiota, Santiago, Chile.
Microbiol Res. 2020 Oct;239:126539. doi: 10.1016/j.micres.2020.126539. Epub 2020 Jun 28.
Clostridioides difficile is a nosocomial, Gram-positive, strictly anaerobic, spore-forming pathogen capable of colonizing and proliferating in the human intestine. In bacteria, it has been shown that the Toxin-Antitoxin systems mediate the cellular response to external stress by initiating processes such as biofilm formation and programmed cell death. This work aims to evaluate the functionality of four type II TA modules of Clostridioides difficile R20291. We performed bioinformatic analysis to search for putative TA systems using the TADB platform. Then we performed a heterologous expression assay to evaluate the functionality of these systems. Our results showed that the MazEF and RelBE systems were functional, suggesting that their corresponding toxins possess an endoribonuclease activity. In conclusion, MazEF and RelBE systems of C. difficile R20291 are functional in a heterologous expression system.
艰难梭菌是一种医院获得性、革兰阳性、严格厌氧、产芽孢的病原体，能够在人类肠道中定植和增殖。在细菌中，已经表明毒素-抗毒素系统通过启动生物膜形成和程序性细胞死亡等过程来介导细胞对外界应激的反应。本工作旨在评估艰难梭菌 R20291 的四个 II 型 TA 模块的功能。我们使用 TADB 平台进行生物信息学分析，以搜索可能的 TA 系统。然后，我们进行了异源表达测定，以评估这些系统的功能。我们的结果表明 MazEF 和 RelBE 系统是有功能的，这表明它们对应的毒素具有内切核糖核酸酶活性。总之，艰难梭菌 R20291 的 MazEF 和 RelBE 系统在异源表达系统中是有功能的。
