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[借助方格网目镜测定组织学标本特定长度的方法]

[Method of determination of specific length of histological specimens with the aid of square-net eyeglass].

作者信息

Kaplan I Ia

出版信息

Arkh Anat Gistol Embriol. 1977;72(1):83-7.

PMID:326231
Abstract

The author has analytically expressed the relationship between the inaccessible for measurement total length of non-randomly oriented stretched objects and the sum of lengths of their projections on the longitudinal axis of the preparation (or another line). The sum of their projection lengths can be measured by means of a square-net ocular. The specific length of objects on a histological section is proportional to the number of their intersections with the transversal lines of the net and the value of its division and inversly proportional to the cosine of the average meaning of angles between the longitudinal axis of the preparation and the objects under study. Verification of the validity of the formula deduced was carried on in the histological section model prepared by the author. The proposed method ensures determination of the specific length of stretched histological preparations with a no more than 3% error.

摘要

作者通过分析得出了不可测量的非随机取向拉伸物体的总长度与其在标本纵轴(或另一条线)上投影长度之和之间的关系。它们投影长度的总和可以通过方格目镜进行测量。组织学切片上物体的比长度与它们与网格横线的交点数量、网格分度值成正比,与标本纵轴和所研究物体之间平均角度余弦值成反比。作者在自制的组织学切片模型上对推导公式的有效性进行了验证。所提出的方法能够确保测定拉伸组织学标本的比长度,误差不超过3%。

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