Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, Műegyetem rkp. 3, H-1111 Budapest, Hungary.
Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, Műegyetem rkp. 3, H-1111 Budapest, Hungary.
Colloids Surf B Biointerfaces. 2020 Oct;194:111219. doi: 10.1016/j.colsurfb.2020.111219. Epub 2020 Jun 24.
Synthetic polyaspartamides with various functional side groups including primary, secondary, tertiary amine or carboxyl groups were designed to explore the effect of chemical composition on polymer-mucin interactions. Since the molecular weight of the polymers and the degree of modification were identical for each derivative, the role of the functional groups could be evaluated. Chitosan was used as a control sample due to its strong interaction with mucin primarily through electrostatic forces. Mucoadhesive interactions of the polymers with the aqueous dispersion of commercially available porcine gastric mucin were probed on the colloid size scale using various methods including turbidimetric titration, dynamic light scattering and zeta potential measurements. Both the charge of the polymers and the type of amine groups had a pronounced effect on the interactions. The interactions were further analysed by partially screening them with either sodium chloride or urea. The results obtained allow us to classify these polymers in terms of in vitro mucoadhesive strength, which can be useful in the design of mucoadhesive formulations.
设计了具有各种官能团的合成聚天冬酰胺，包括伯胺、仲胺、叔胺或羧基，以探索化学成分对聚合物-粘蛋白相互作用的影响。由于每种衍生物的聚合物分子量和修饰程度都相同，因此可以评估官能团的作用。壳聚糖被用作对照样品，因为它主要通过静电力与粘蛋白强烈相互作用。使用各种方法，包括浊度滴定、动态光散射和zeta 电位测量，在胶体尺寸范围内探测聚合物与市售猪胃粘蛋白水相分散体的粘附相互作用。聚合物的电荷和胺基类型对相互作用都有显著影响。通过用氯化钠或尿素部分屏蔽这些相互作用，进一步对其进行了分析。所得结果允许我们根据体外粘合力对这些聚合物进行分类，这在设计粘合力制剂时可能很有用。
