College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Joint International Research Laboratory of Animal Health and Food Safety, National Experimental Teaching Demonstration Center of Animal Science, Nanjing Agricultural University, Nanjing, P. R. China.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, P. R. China.
J Sci Food Agric. 2021 Jan 15;101(1):279-286. doi: 10.1002/jsfa.10641. Epub 2020 Aug 4.
Wooden breast (WB) myopathy in broiler chickens is a growing challenge for the poultry industry. Previous multi-omic data have implied that the pathogenesis of WB is associated with the activation of immune system and inflammatory response. However, the intricate mechanisms are not fully understood. This study was therefore conducted to systematically investigate the morphology, expression of cytokines as well as the underlying signaling pathways regulating the inflammatory response in pectoralis major (PM) muscle of WB myopathic broilers.
wHistopathological changes, increased plasma creatine kinase and lactate dehydrogenase activities, elevated myeloperoxidase activity and overproduction of nitric oxide in muscle indicated the enhancement of muscle damage and inflammation in WB broilers. The messenger RNA (mRNA) expressions of inflammatory cytokines were dysregulated in PM muscle and contents of interleukin (IL)-1β, IL-8 and tumor necrosis factor-α were increased in serum of WB myopathic broilers, indicating this myopathy was associated with immune disorder and systemic inflammation response. Additionally, toll-like receptor (TLR) levels were upregulated, the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway was activated and the mRNA expression levels of downstream inflammatory mediators were enhanced in PM muscle of WB myopathy affected birds.
The results indicated an immune disorder and a systemic inflammation response in WB myopathic broilers, which might be related to a synergetic effect of TLRs and NF-κB pathway. © 2020 Society of Chemical Industry.
木质鸡胸（WB）肉鸡肌病是家禽业面临的一个日益严峻的挑战。先前的多组学数据表明，WB 的发病机制与免疫系统的激活和炎症反应有关。然而，其复杂的机制尚未完全阐明。因此，本研究旨在系统研究 WB 肌病肉鸡 PM 肌肉中的形态学、细胞因子表达以及调节炎症反应的潜在信号通路。
WB 肉鸡的肌肉组织出现组织病理学变化，血浆肌酸激酶和乳酸脱氢酶活性升高，髓过氧化物酶活性升高，一氧化氮生成增加，表明肌肉损伤和炎症加重。PM 肌肉中炎症细胞因子的信使 RNA（mRNA）表达失调，WB 肌病肉鸡血清中白细胞介素（IL）-1β、IL-8 和肿瘤坏死因子-α的含量增加，表明这种肌病与免疫紊乱和全身炎症反应有关。此外，WB 肌病肉鸡的 TLR 水平上调，核因子 kappa-轻链增强子的 B 细胞（NF-κB）信号通路被激活，下游炎症介质的 mRNA 表达水平增强。
这些结果表明 WB 肌病肉鸡存在免疫紊乱和全身炎症反应，这可能与 TLR 和 NF-κB 通路的协同作用有关。 © 2020 英国化学学会。
