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低氮需求酵母菌株的筛选及其对蜂蜜酒理化和挥发性成分的影响。

Selection of low nitrogen demand yeast strains and their impact on the physicochemical and volatile composition of mead.

作者信息

Schwarz Luisa Vivian, Marcon Angela Rossi, Delamare Ana Paula Longaray, Agostini Fabiana, Moura Sidnei, Echeverrigaray Sergio

机构信息

Institute of Biotechnology, University of Caxias Do Sul (UCS), Francisco Getúlio Vargas 1130, Caxias do Sul, RS 95070-560 Brazil.

Federal University of Pampa (UNIPAMPA), 21 de Abril 80, Dom Pedrito, RS 96450-000 Brazil.

出版信息

J Food Sci Technol. 2020 Aug;57(8):2840-2851. doi: 10.1007/s13197-020-04316-6. Epub 2020 Mar 2.

Abstract

Mead is an ancient alcoholic beverage produced through the fermentation of a diluted solution of honey. Due to the peculiar and varied composition of honey, mead production faces several problems, such as slow or stuck fermentations mainly due to the low nitrogen concentration, lack of uniformity of the final product and the production of unpleasant aromas. In this context, this work aimed to select low nitrogen-demand yeast strains and evaluate their potential for the production of mead. Therefore, among 21 commercial wine yeast strains, 5 were selected based on their fermentative behavior at low assimilable nitrogen concentrations. The selected strains were further evaluated for their contributions in meads produced with limited nitrogen availability, and the results showed significant differences on some physicochemical parameters like biomass production, residual sugars, glycerol concentration, and fermentative rate. Moreover, meads obtained with selected strains differed in the concentration of several volatile compounds. The volatile compounds concentration and the principal component analysis based on odor activity values allowed separating strains into three groups. In general, var strains (QA23, Spark, and AWRI-R2) were the largest producers of aromatic compounds, particularly those with floral and fruity descriptors. The selection of yeast strains with low nitrogen-demand and different volatile compounds production can be explored by mead makers to limit fermentation problems and obtain characteristic products.

摘要

蜂蜜酒是一种通过蜂蜜稀释溶液发酵制成的古老酒精饮料。由于蜂蜜独特且多样的成分,蜂蜜酒生产面临若干问题,例如主要由于氮浓度低导致发酵缓慢或停滞、最终产品缺乏一致性以及产生令人不悦的香气。在此背景下,本研究旨在筛选低氮需求酵母菌株并评估其生产蜂蜜酒的潜力。因此,在21种商业葡萄酒酵母菌株中,根据它们在低可同化氮浓度下的发酵行为选择了5种。对所选菌株在氮供应有限的情况下生产的蜂蜜酒中的贡献进行了进一步评估,结果显示在一些理化参数上存在显著差异,如生物量生产、残糖、甘油浓度和发酵速率。此外,用所选菌株获得的蜂蜜酒在几种挥发性化合物的浓度上有所不同。基于气味活性值的挥发性化合物浓度和主成分分析将菌株分为三组。一般来说,var菌株(QA23、Spark和AWRI-R2)是芳香化合物的最大生产者,特别是那些具有花香和果香描述的化合物。蜂蜜酒制造商可以探索选择低氮需求和产生不同挥发性化合物的酵母菌株,以限制发酵问题并获得具有特色的产品。

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