Cetmi Serife Dilara, Renkler Nergis Zeynep, Kose Ayse, Celik Cenk, Oncel Suphi S
Department of Bioengineering Faculty of Engineering, Ege University Bornova Izmir Turkey.
Yong Loo Lin School of Medicine National University of Singapore Singapore.
Eng Life Sci. 2019 Aug 22;19(10):691-699. doi: 10.1002/elsc.201900009. eCollection 2019 Oct.
Sustainable, ecological, and biocompatible materials are emerging for the development of novel components for tissue engineering. Microalgae being one of the unique organisms on Earth to provide various novel compounds with certain bioactivities are also a good source for the development of novel tissue scaffold materials. In this study, electrospinning technique was utilized to fabricate nanofibers from polycaprolactone loaded with microalgal extracts obtained from (vegetative and carotenoid producing form) and . The FTIR results showed that, blending microalgae with polycaprolactone give unique bands rooted from microalgae and polycaprolactone structure. The samples were not diversified from each other, however stable bands were observed. SEM analysis revealed a uniform fiber fabrication with an average diameter of 810 ± 55 nm independent from microalgal extracts. MTT assay was done on HUVEC cell lines and results showed that nanofiber mats helped cell proliferation with extended time. Biodegradation resulted with mineral accumulation on the surface of same samples however the fiber degradation was uniform. With slow but stable biodegradation characteristics, microalgal extract loaded nanofiber mats holds great potential to be novel tissue scaffold material.
可持续、生态且生物相容的材料正在涌现，用于开发组织工程的新型组件。微藻作为地球上能够提供各种具有特定生物活性的新型化合物的独特生物体之一，也是开发新型组织支架材料的良好来源。在本研究中，采用静电纺丝技术，以负载有从（营养型和产类胡萝卜素型）和中获得的微藻提取物的聚己内酯制备纳米纤维。傅里叶变换红外光谱（FTIR）结果表明，将微藻与聚己内酯混合产生了源自微藻和聚己内酯结构的独特谱带。样品之间没有差异，但观察到了稳定的谱带。扫描电子显微镜（SEM）分析显示，独立于微藻提取物，平均直径为810±55纳米的纤维制备均匀。对人脐静脉内皮细胞（HUVEC）系进行了MTT试验，结果表明纳米纤维垫在延长的时间内有助于细胞增殖。生物降解导致相同样品表面有矿物质积累，但纤维降解是均匀的。由于具有缓慢但稳定的生物降解特性，负载微藻提取物的纳米纤维垫具有成为新型组织支架材料的巨大潜力。
