Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China.
Biomed Res Int. 2020 Jun 11;2020:8542797. doi: 10.1155/2020/8542797. eCollection 2020.
The section is one of the components of the genus subgenus (Amaryllidaceae), and species relationship in this section is still not resolved very well, which hinders further evolutionary and adaptive studies. Here, the complete chloroplast genomes of five sect. species were reported, and a comparative analysis was performed with other three related species. The genome size of the eight species ranged from 151,672 bp to 153,339 bp in length, GC content changed from 36.7% to 36.8%, and 130 genes (except ), 37 tRNA, and 8 rRNA were identified in each genome. By analyzing the IR/LSC and IR/SSC boundary, exhibited differences compared with other seven species. Phylogenetic analysis achieved high supports in each branch, seven of the eight species cluster into a group, and exhibit a close relationship with . Higher pairwise Ka/Ks ratios were found in compared to and while a lower value of Ka/Ks ratios was detected between and . This study will be a great contribution to the future phylogenetic and adaptive research in .
该节是（Amaryllidaceae）属（subgenus ）的一个组成部分，该节中物种的关系还没有很好地解决，这阻碍了进一步的进化和适应性研究。在这里，报道了五个节（sect. ）物种的完整叶绿体基因组，并与其他三个相关物种进行了比较分析。八个物种的基因组大小在 151672bp 到 153339bp 之间，GC 含量在 36.7%到 36.8%之间，每个基因组都鉴定出了 130 个基因（除了），37 个 tRNA 和 8 个 rRNA。通过分析 IR/LSC 和 IR/SSC 边界，与其他七个物种相比，表现出了差异。系统发育分析在每个分支中都得到了高度支持，八个中的七个（Amaryllidaceae）物种聚集成一个组，与（Hymenocallis）关系密切。与（Hymenocallis）和（Fritillaria）相比，在（Leucojum）中发现了更高的 Ka/Ks 比值，而在（Leucojum）和（Fritillaria）之间检测到了更低的 Ka/Ks 比值。这项研究将对未来（Amaryllidaceae）属的系统发育和适应性研究做出巨大贡献。
