Oprins A C, Fentener van Vlissingen J M, Blankenstein M A
Department of Anatomy, School of Veterinary Medicine, University of Utrecht, The Netherlands.
J Steroid Biochem. 1988 Oct;31(4A):387-91. doi: 10.1016/0022-4731(88)90306-8.
We have adapted an oil microcentrifuge assay for evaluating the binding of tritiated methyltrienolone ([3H]R1881) in metabolically active primary cultured porcine gubernaculum fibroblasts. Almost complete separation of cells with the bound hormone and medium with the unbound hormone can be achieved within 30 s of centrifugation at 10,000 g. Specific [3H]R1881 binding was found in four different gubernaculum fibroblast cultures (9,000-42,000 sites/cell) and MCF-7 human breast cancer cells (34,000 sites/cell) which were used as controls. Binding was inhibited by radio-inert R1881 and 5 alpha-dihydrotestosterone but not by estradiol and cortisol. The results indicate that androgen receptors are present in fibroblasts from the gubernaculum during both the first and second phase of testicular descent and that they may play a role in the regulation of that process.
我们采用了一种油微量离心测定法，用于评估氚标记的甲基三烯醇酮（[3H]R1881）在代谢活跃的原代培养猪睾丸引带成纤维细胞中的结合情况。在10,000 g离心30秒内，几乎可以将结合了激素的细胞与未结合激素的培养基完全分离。在四种不同的睾丸引带成纤维细胞培养物（9,000 - 42,000个位点/细胞）以及用作对照的MCF - 7人乳腺癌细胞（34,000个位点/细胞）中发现了特异性的[3H]R1881结合。结合受到放射性惰性的R1881和5α - 二氢睾酮的抑制，但不受雌二醇和皮质醇的抑制。结果表明，在睾丸下降的第一阶段和第二阶段，睾丸引带中的成纤维细胞均存在雄激素受体，并且它们可能在该过程的调节中发挥作用。
