Faruk Md Shohel Al, Jung Young-Hoon, Hur Tai-Young, Lee Sang-Suk, Cho Yong-Il
Department of Animal Science and Technology, Sunchon National University, Suncheon, Jeonnam 57922, Korea.
National Institute of Animal Science, Cheonan, Chungnam 3100, Korea.
Vet Sci. 2020 Jun 30;7(3):81. doi: 10.3390/vetsci7030081.
subsp. (MAP) is the causative agent of Johne's disease in dairy cattle populations around the world. The objective of this study was to evaluate MAP antibody kinetics in serum and milk samples throughout the lactation period in dairy cattle. The samples were collected simultaneously from eight MAP-positive and two healthy MAP-negative (control group) cows. The MAP antibody was detected by using serum and milk ELISA. The serum and milk MAP antibody titers fluctuated between the positive and negative cut-off values in this study. Specifically, cattle with low MAP antibody titer (<100) showed fluctuation between the cut-off values. Variable changes of MAP antibody titer were also observed after parturition. Between the serum and milk MAP antibody titers, there was a positive correlation (R = 0.5358) observed throughout the assessment period. The milk MAP ELISA test had low diagnostic performance in cows with low MAP titer due to its weak correlation (R = 0.0198). Finally, this study suggest that the periodic MAP ELISA test is recommended for the application of Johne's eradication program due to the fluctuating nature of MAP antibody kinetics.
亚种（MAP）是全球奶牛群体中约内氏病的病原体。本研究的目的是评估奶牛整个泌乳期血清和乳样中MAP抗体的动力学变化。同时从8头MAP阳性奶牛和2头健康的MAP阴性（对照组）奶牛采集样本。采用血清和乳ELISA法检测MAP抗体。在本研究中，血清和乳MAP抗体滴度在阳性和阴性临界值之间波动。具体而言，MAP抗体滴度低（<100）的奶牛其滴度在临界值之间波动。分娩后也观察到MAP抗体滴度的变化。在整个评估期内，血清和乳MAP抗体滴度之间存在正相关（R = 0.5358）。由于相关性较弱（R = 0.0198），乳MAP ELISA检测在MAP滴度低的奶牛中诊断性能较低。最后，本研究表明，由于MAP抗体动力学的波动特性，建议在约内氏病根除计划中定期进行MAP ELISA检测。
