Xu Qianqian, Li Huaiyu, Zhou Wenting, Zou Xiaoting, Dong Xinyang
Key Laboratory for Molecular Animal Nutrition of Ministry of Education, College of Animal Sciences, Zhejiang University (Zijingang Campus), Hangzhou 310058, China.
Animals (Basel). 2020 Jul 1;10(7):1121. doi: 10.3390/ani10071121.
The objective of this study was to evaluate the age-related changes in antioxidant status and the lipid metabolism of pigeon squabs (), by determining the BW, antioxidant indices, serum lipid levels, lipid metabolism-related enzyme activities, lipid metabolism-related gene expression, and liver morphology in squabs. Ten squabs were randomly selected and sampled on the day of hatching (DOH), days 7 (D7), 14 (D14) and 21 (D21) post-hatch, respectively. The results showed that BW of squabs increased linearly from DOH to D21. The minimum fold of BW gain was observed in the phase from D14 to D21. Serum triglyceride and free fatty acid levels displayed linear and quadratic trends as age increased, with these maximum responses in D14. Serum low-density lipoprotein cholesterol level responded to age linearly and quadratically with the minimum in D14. Serum high-density lipoprotein cholesterol level and the ratio of high-density lipoprotein cholesterol to low-density lipoprotein cholesterol increased linearly with age, whereas the very low-density lipoprotein cholesterol level decreased linearly. The activities of glutathione peroxidase, catalase, and superoxide dismutase in liver displayed linear and quadratic trends as age increased, with these minimum responses in D14. Hepatic malondialdehyde concentration responded to age linearly and quadratically, with the maximum in D14. Activities of lipoprotein lipase, hepatic lipase, and 3-hydroxy-3-methyl glutaryl coenzyme A reductase in liver responded to age linearly and quadratically, with these minimum responses in D14. Hepatic hormone-sensitive lipase activity displayed linear and quadratic trends as age increased with the maximum in D14. Hepatic acetyl CoA carboxylase activity on D14 was significantly lower than squabs on DOH and D7. Hepatic carnitine palmitoyltransferase 1 mRNA expression responded to age linearly and quadratically, with minimum response in D14. Hepatic mRNA expression of acetyl CoA carboxylase and fatty acid synthetase increased linearly with age. Hepatic Oil-Red-O staining area displayed a quadratic trend as age increased, with the maximum response in D14. In conclusion, the phase from DOH to D14 was a crucial development stage for growth, antioxidant status and lipid metabolism in pigeon squabs. The results suggest it is better to take nutritional manipulation in squabs before D14.
本研究的目的是通过测定乳鸽的体重、抗氧化指标、血脂水平、脂质代谢相关酶活性、脂质代谢相关基因表达以及肝脏形态，评估乳鸽抗氧化状态和脂质代谢的年龄相关变化。分别在孵化日（DOH）、孵化后第7天（D7）、14天（D14）和21天（D21）随机选取10只乳鸽并进行采样。结果表明，乳鸽的体重从DOH到D21呈线性增加。在D14至D21阶段观察到体重增加倍数最小。血清甘油三酯和游离脂肪酸水平随年龄增长呈线性和二次趋势变化，在D14时达到最大值。血清低密度脂蛋白胆固醇水平随年龄呈线性和二次反应，在D14时最低。血清高密度脂蛋白胆固醇水平以及高密度脂蛋白胆固醇与低密度脂蛋白胆固醇的比值随年龄呈线性增加，而极低密度脂蛋白胆固醇水平呈线性下降。肝脏中谷胱甘肽过氧化物酶、过氧化氢酶和超氧化物歧化酶的活性随年龄增长呈线性和二次趋势变化，在D14时反应最低。肝脏丙二醛浓度随年龄呈线性和二次反应，在D14时最高。肝脏中脂蛋白脂肪酶、肝脂酶和3-羟基-3-甲基戊二酰辅酶A还原酶的活性随年龄呈线性和二次反应，在D14时反应最低。肝脏激素敏感性脂肪酶活性随年龄增长呈线性和二次趋势变化，在D14时最高。D14时肝脏乙酰辅酶A羧化酶活性显著低于DOH和D7时的乳鸽。肝脏肉碱棕榈酰转移酶1 mRNA表达随年龄呈线性和二次反应，在D14时反应最低。肝脏乙酰辅酶A羧化酶和脂肪酸合成酶的mRNA表达随年龄呈线性增加。肝脏油红O染色面积随年龄增长呈二次趋势变化，在D14时反应最大。总之，从DOH到D14阶段是乳鸽生长、抗氧化状态和脂质代谢的关键发育阶段。结果表明，最好在D14之前对乳鸽进行营养调控。
