Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan 250023, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271018, China.
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271018, China.
Poult Sci. 2022 May;101(5):101797. doi: 10.1016/j.psj.2022.101797. Epub 2022 Feb 24.
The purpose of this study was to investigate whether in ovo feeding of t10,c12-conjugated linoleic acid (CLA) could regulate hepatic lipid metabolism and decrease lipid accumulation in newly hatched chicks. Three hundred and sixty fertilely specific pathogen-free hatching eggs were selected and randomly divided into 6 groups. On embryonic day 11 of incubation (E11), 0, 1.5, 3.0, 4.5, 6.0, or 7.5 mg t10,c12-CLA were injected into the eggs. The results indicated that in ovo feeding of t10,c12-CLA significantly decreased the subcutaneous adipose tissue (SAT) mass and the relative SAT weight of newly hatched chicks in linear and quadratic manners (P < 0.05). In liver, the levels of triglycerides were reduced linearly and quadratically and total cholesterol were reduced quadratically as the dose of t10,c12-CLA increased (P < 0.05). Meanwhile, the hepatic carnitine palmitoyltransferase-1a (CPT1a) content and polyunsaturated fatty acid proportion were increased quadratically in t10,c12-CLA groups (P < 0.05), accompanied by the decrease of malondialdehyde level and the increase of glutathione peroxidase and total antioxidant capacity activities (P < 0.05). In addition, in ovo feeding of t10,c12-CLA decreased the mRNA expression levels of fatty acid synthase, acetyl-CoA carboxylase 1 in linear and quadratic manners (P < 0.05), and decreased the mRNA expression of adipose triacylglyceride lipase and stearoyl-CoA desaturase significantly in liver (P < 0.05), accompanied by upregulating the mRNA expression of CPT1a quadratically and AMP-activated protein kinase α linearly and quadratically (P < 0.05). In SAT, the mRNA expression of peroxisome proliferator-activated receptor γ (PPARγ) and sterol regulatory element-binding protein-1c were decreased linearly and quadratically (P < 0.05), and the expression of PPARα and CPT1a genes were increased linearly and quadratically as the dose of t10,c12-CLA increased (P < 0.05). In conclusion, our findings demonstrate that in ovo feeding of t10,c12-CLA alleviates lipid accumulation in newly hatched chicks by suppressing fatty acid synthesis and stimulating lipolysis in the liver and inhibiting adipocyte differentiation in subcutaneous adipose tissue.
本研究旨在探讨在鸡胚期饲喂反式-10，顺式-12 共轭亚油酸（CLA）是否可以调节肝脏脂质代谢并减少刚孵化小鸡的脂质积累。选择 360 枚无菌受精种蛋并随机分为 6 组。在孵化第 11 天（E11），向蛋中注射 0、1.5、3.0、4.5、6.0 或 7.5mg t10，c12-CLA。结果表明，鸡胚期饲喂 t10，c12-CLA 可显著降低刚孵化小鸡的皮下脂肪组织（SAT）质量和相对 SAT 重量，呈线性和二次关系（P<0.05）。在肝脏中，随着 t10，c12-CLA 剂量的增加，甘油三酯水平呈线性和二次降低，总胆固醇呈二次降低（P<0.05）。同时，t10，c12-CLA 组肝肉毒碱棕榈酰转移酶-1a（CPT1a）含量和多不饱和脂肪酸比例呈二次增加（P<0.05），丙二醛水平降低，谷胱甘肽过氧化物酶和总抗氧化能力活性增加（P<0.05）。此外，鸡胚期饲喂 t10，c12-CLA 可呈线性和二次降低脂肪酸合成酶和乙酰辅酶 A 羧化酶 1 的 mRNA 表达水平（P<0.05），并显著降低肝脏中脂肪甘油三酯脂肪酶和硬脂酰辅酶 A 去饱和酶的 mRNA 表达水平（P<0.05），同时呈二次增加 CPT1a 和线性和二次增加 AMP 激活的蛋白激酶α的 mRNA 表达水平（P<0.05）。在 SAT 中，过氧化物酶体增殖物激活受体γ（PPARγ）和固醇调节元件结合蛋白-1c 的 mRNA 表达呈线性和二次降低（P<0.05），随着 t10，c12-CLA 剂量的增加，PPARα 和 CPT1a 基因的表达呈线性和二次增加（P<0.05）。综上所述，本研究结果表明，在鸡胚期饲喂 t10，c12-CLA 通过抑制脂肪酸合成和刺激肝脏中的脂肪分解以及抑制皮下脂肪组织中的脂肪细胞分化，减轻刚孵化小鸡的脂质积累。
