Hansen J T, Kordower J H, Fiandaca M S, Jiao S S, Notter M F, Gash D M
Department of Neurobiology and Anatomy, University of Rochester School of Medicine and Dentistry, New York 14642.
Exp Neurol. 1988 Oct;102(1):65-75. doi: 10.1016/0014-4886(88)90079-9.
Based largely upon studies done in rats, a number of medical centers are now performing autografts of adrenal medullary tissue in consenting patients with Parkinson's disease. However, a systematic experimental evaluation of adrenal medullary autografts in nonhuman primates is necessary. This study provides a detailed analysis of the implant site at the fine structural level 30 days post-transplantation in the Cebus monkey. Five normal and two 1-methyl-4-phenyl-1,2,5,6-tetrahydropyridine (MPTP)-treated Cebus monkeys received adrenal medullary autografts using an open microsurgical approach (n = 3) or via stereotactic placement with a tissue carrier (n = 4). Analysis of preimplant samples of the adrenal medulla confirmed that viable chromaffin cells were implanted into the basal ganglia. However, 30 days later, the implant site resembled a chronic inflammatory focus, with grafted chromaffin cells identified ultrastructurally in only two of the seven transplanted monkeys. The grafted cells showed overt signs of cellular degeneration and were surrounded by phagocytic macrophages. All of the implant sites, regardless of the surgical approach, were filled with macrophages, cells of hematogenous origin, and fibrous astrocytes. The vasculature of the implant site was of the nonfenestrated type, characteristic of the host striatum. Despite the poor survival of implanted chromaffin cells, robust sprouting of tyrosine hydroxylase-like immunoreactive fibers was evident in the striatum adjacent to the implant site (see accompanying manuscript, M.S. Fiandaca, J. H. Kordower, J.T. Hansen, S.-S. Jiao, and D.M. Gash, 1988, Exp. Neurol. 102: 76-91), suggesting that implantation may have precipitated a host response that was beneficial to the transplanted animal. Additional studies that provide a better understanding of the cellular elements residing in the implant site and their potential for trophic influence seem warranted.
基于在大鼠身上所做的大量研究，一些医疗中心目前正在对患有帕金森病且表示同意的患者进行肾上腺髓质组织自体移植。然而，对非人类灵长类动物的肾上腺髓质自体移植进行系统的实验评估是必要的。本研究对移植后30天的僧帽猴植入部位进行了精细结构水平的详细分析。五只正常僧帽猴和两只经1-甲基-4-苯基-1,2,5,6-四氢吡啶（MPTP）处理的僧帽猴接受了肾上腺髓质自体移植，其中三只采用开放显微手术方法，四只通过带有组织载体的立体定向植入。对肾上腺髓质植入前样本的分析证实，有活力的嗜铬细胞被植入到基底神经节。然而，30天后，植入部位类似慢性炎症病灶，在七只移植猴中只有两只通过超微结构鉴定出移植的嗜铬细胞。移植细胞显示出明显的细胞变性迹象，并被吞噬性巨噬细胞包围。所有植入部位，无论手术方法如何，都充满了巨噬细胞、血源性细胞和纤维性星形胶质细胞。植入部位的血管属于无窗型，这是宿主纹状体的特征。尽管植入的嗜铬细胞存活率很低，但在植入部位相邻的纹状体中，酪氨酸羟化酶样免疫反应性纤维有明显的大量增生（见随附论文，M.S. 菲安达卡、J.H. 科尔道尔、J.T. 汉森、S.-S. 焦和D.M. 加什，1988年，《实验神经病学》102: 76 - 91），这表明植入可能引发了对移植动物有益的宿主反应。似乎有必要进行更多研究，以更好地了解植入部位的细胞成分及其营养影响潜力。
