植物中转高尔基网络囊泡的分离与糖基分析。

Isolation and Glycomic Analysis of Trans-Golgi Network Vesicles in Plants.

机构信息

Department of Plant Sciences, University of California, Davis, CA, USA.

School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.

出版信息

Methods Mol Biol. 2020;2177:153-167. doi: 10.1007/978-1-0716-0767-1_13.

DOI:10.1007/978-1-0716-0767-1_13

PMID:32632812

Abstract

The dynamic endomembrane system facilitates sorting and transport of diverse cargo. Therefore, it is crucial for plant growth and development. Vesicle proteomic studies have made substantial progress in recent years. In contrast, much less is known about the identity of vesicle compartments that mediate the transport of polysaccharides to and from the plasma membrane and the types of sugars they selectively transport. In this chapter, we provide a detailed description of the protocol used for the elucidation of the SYP61 vesicle population glycome. Our methodology can be easily adapted to perform glycomic studies of a broad variety of plant cell vesicle populations defined via subcellular markers or different treatments.

摘要

动态内膜系统有助于对不同货物进行分类和运输。因此，它对植物的生长和发育至关重要。近年来，囊泡蛋白质组学研究取得了重大进展。相比之下，人们对介导多糖在质膜内外运输的囊泡隔室的身份以及它们选择性运输的糖的类型知之甚少。在本章中，我们提供了详细描述阐明 SYP61 囊泡群体聚糖组的方案。我们的方法可以很容易地适应通过亚细胞标记物或不同处理来定义的各种植物细胞囊泡群体的糖组学研究。