Boehringer Ingelheim Animal Health, Saint-Priest, France.
Methods Mol Biol. 2020;2134:31-40. doi: 10.1007/978-1-0716-0459-5_4.
Rapid and reliable enumeration of Leptospira spp., the causative agent of leptospirosis, represents a technical challenge because leptospires are thin, highly motile, and slow-growing bacteria. The current gold standard for cell enumeration is the use of a Petroff-Hausser counting chamber and a dark-field microscope, but this method remains time-consuming and lacks reproducibility. New alternative techniques are then of great interest. Here we describe the protocol for counting leptospires by flow cytometry. This method is rapid, reproducible, sensitive, and hence suitable to become a new standard to enumerate Leptospira spp.
快速可靠地计数钩端螺旋体属（Leptospira spp.），即钩端螺旋体病的病原体，是一项技术挑战，因为钩端螺旋体是细长、高度运动和缓慢生长的细菌。目前，细胞计数的金标准是使用 Petroff-Hausser 计数室和暗场显微镜，但这种方法仍然耗时且缺乏重现性。因此，新的替代技术非常有意义。本文描述了使用流式细胞术计数钩端螺旋体的方案。该方法快速、可重复、灵敏，因此适合成为一种新的标准来计数钩端螺旋体属。
