Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Laboratory of Bacteriology, Butantan Institute, São Paulo, Brazil.
Methods Mol Biol. 2020;2134:187-198. doi: 10.1007/978-1-0716-0459-5_17.
Like many other pathogens of medical importance, pathogenic Leptospira employ diverse strategies to circumvent Complement System activation. Under physiological conditions, this central humoral arm of innate immunity is tightly controlled by negative Complement regulatory proteins. However, upon infection, pathogenic microorganisms interfere with normal Complement host defense mechanisms by recruiting or mimicking Complement regulators and by secreting endogenous proteases or acquiring host's proteases that inactivate key Complement components. In this chapter, we describe in detail some of the most frequently used assays to evaluate Leptospira Complement resistance.
与许多其他具有医学重要性的病原体一样，致病性钩端螺旋体采用多种策略来规避补体系统的激活。在生理条件下，这种先天免疫的核心体液臂受到阴性补体调节蛋白的严格控制。然而，在感染期间，致病性微生物通过招募或模拟补体调节剂以及分泌内源性蛋白酶或获得使关键补体成分失活的宿主蛋白酶，干扰正常的补体宿主防御机制。在本章中，我们详细描述了一些最常用于评估钩端螺旋体补体抗性的常用测定方法。
