Ghio R, Pistoia V, Romagnoli M, D'Elia P, Boccaccio G P
Cattedra di Terapia Medica, Universitá di Genova, Italy.
Int J Immunopharmacol. 1988;10(3):237-45. doi: 10.1016/0192-0561(88)90054-9.
The mechanisms underlying the inhibition of T-cell proliferation by methylprednisolone (MPS) have been investigated using a T-cell colony assay. Cell fractionation experiments have demonstrated that MPS exerted its effects at the level of both T-cells and monocytes. Thus, monocytes treated with MPS released a soluble factor that had suppressor activity on T-cell proliferation. Moreover, MPS directly blocked the proliferative capacity of T-cells, as demonstrated by the finding that MPS-treated T-cells formed reduced numbers of colonies even under optimal culture conditions and in the presence of exogenous IL-2.
已使用T细胞集落测定法研究了甲基强的松龙（MPS）抑制T细胞增殖的潜在机制。细胞分级分离实验表明，MPS在T细胞和单核细胞水平上均发挥作用。因此，用MPS处理的单核细胞释放出一种对T细胞增殖具有抑制活性的可溶性因子。此外，MPS直接阻断T细胞的增殖能力，这一发现表明，即使在最佳培养条件下且存在外源性白细胞介素-2的情况下，经MPS处理的T细胞形成的集落数量也会减少。
