Zhu Q-Q, Tian L, Li D-L, Wu Z-H, He Y-Y, Zhang H-K
Department of Vascular Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Eur Rev Med Pharmacol Sci. 2020 Jun;24(12):6597-6604. doi: 10.26355/eurrev_202006_21645.
To explore roles of CDGSH iron-sulfur domain-containing protein 2 (CISD2) in the progression of prostate cancer (PCa) cells, and relationships between CISD2 expression and the prognosis and clinical pathological parameters in PCa patients.
Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) analysis and Western blot analysis were used to detect the CISD2 expression in PCa tissues and cells. CISD2 siRNA was used to inhibit the CISD2 expression. Kaplan-Meier method and Log rank analysis were performed to determine survival analysis while Chi-square test was performed to analyze the association between CISD2 and clinicopathological parameters of PCa patients. Transwell assay and wound healing assay was conducted to examine the invasion and migration ability of PCa cells, respectively.
CISD2 was up-regulated in PCa tissues and cells, and showed positive association with the poor prognosis, T stage, lymphatic invasion, prostate-specific antigen (PSA) level, and distant metastasis of PCa patients. Besides, we found that inhibition of CISD2 significantly impaired the migration and invasion ability of PCa cells.
The paper demonstrated that CISD2 could act as a new target for the diagnosis and treatment of PCa patients.
探讨含CDGSH铁硫结构域蛋白2（CISD2）在前列腺癌细胞进展中的作用，以及CISD2表达与前列腺癌（PCa）患者预后和临床病理参数之间的关系。
采用定量实时聚合酶链反应（qRT-PCR）分析和蛋白质印迹分析检测PCa组织和细胞中CISD2的表达。使用CISD2小干扰RNA（siRNA）抑制CISD2表达。采用Kaplan-Meier法和Log rank分析进行生存分析，同时采用卡方检验分析CISD2与PCa患者临床病理参数之间的相关性。分别进行Transwell实验和伤口愈合实验检测PCa细胞的侵袭和迁移能力。
CISD2在PCa组织和细胞中上调，且与PCa患者的不良预后、T分期、淋巴转移、前列腺特异性抗原（PSA）水平及远处转移呈正相关。此外，我们发现抑制CISD2可显著削弱PCa细胞的迁移和侵袭能力。
本文表明CISD2可作为PCa患者诊断和治疗的新靶点。
