Infectious Disease & Molecular Biology Laboratory, Chongqing Medical University, Chongqing, China.
Eur Rev Med Pharmacol Sci. 2020 Jun;24(12):6657-6664. doi: 10.26355/eurrev_202006_21652.
The aim of this study was to explore the expression manuals of microRNA-593 (miR-593) in hepatocellular carcinoma (HCC), and to investigate its role in mediating the proliferation, migration and invasion of HCC cells.
Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect miR-593 expression in HCC tissues and cells. HCC cells were, then, transfected with miR-593 mimic to exogenously overexpress miR-593. Cell counting kit 8 (CCK8) assay was exploited to detect the proliferation ability of HCC cells. In addition, wound healing assay and transwell assay were adopted to determine the migration and invasion abilities of HCC cells, respectively.
MiR-593 was abnormally down-expressed in HCC tissues and cells. Up-regulation of miR-593 significantly inhibited the proliferation, migration and invasion of HCC cells.
MiR-593 acted as a tumor suppressor gene in the development of HCC. Hence, we proposed that miR-593 might be a new potential therapeutic target marker for HCC treatment.
本研究旨在探讨 microRNA-593（miR-593）在肝细胞癌（HCC）中的表达规律，并研究其在介导 HCC 细胞增殖、迁移和侵袭中的作用。
采用实时定量聚合酶链反应（RT-qPCR）检测 HCC 组织和细胞中 miR-593 的表达。然后，用 miR-593 模拟物转染 HCC 细胞以过表达 miR-593。细胞计数试剂盒 8（CCK8）检测 HCC 细胞的增殖能力。此外，采用划痕愈合实验和 Transwell 实验分别测定 HCC 细胞的迁移和侵袭能力。
miR-593 在 HCC 组织和细胞中异常低表达。miR-593 的上调显著抑制了 HCC 细胞的增殖、迁移和侵袭。
miR-593 在 HCC 的发生发展中起抑癌基因的作用。因此，我们提出 miR-593 可能是 HCC 治疗的新的潜在治疗靶点标志物。
