Department of Respiratory Medicine, Yantaishan Hospital, Yantai, China.
Eur Rev Med Pharmacol Sci. 2020 Jun;24(12):6752-6758. doi: 10.26355/eurrev_202006_21663.
To explore the effects of long intergenic non-coding ribonucleic acid 00460 (LINC00460) on the proliferation and apoptosis of non-small cell lung cancer (NSCLC) cells.
In this study, the expression of LINC00460 in lung cancer tissues and its prognostic potential in NSCLC patients were analyzed using the Gene Expression Profiling Interactive Analysis (GEPIA) website and the Cancer Genome Atlas (TCGA) database. Then, the influences of silenced LINC00460 on proliferation and apoptosis in A549 cells were observed via methyl thiazolyl tetrazolium (MTT) assay, colony formation assay, and flow cytometry in vitro. Moreover, Dual-Luciferase reporter assay and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) were performed to detect the targeting relationship between LINC00460 and micro RNA (miR)-539. The biological role of the LINC00460/miR-539 axis in the proliferation of A549 cells was examined using MTT assay.
It was found that the expression level of LINC00460 was significantly upregulated in NSCLC tissues and cell lines, and particularly negatively correlated with the overall survival (OS). According to the in vitro experimental results, LINC00460 knockdown inhibited the proliferation of A549 cells, but promoted their apoptosis. Dual-Luciferase reporter assay results revealed that miR-539 was directly targeted by LINC00460 and involved in the LINC00460-mediated proliferation of A549 cells.
LINC00460 is overexpressed in NSCLC tissues and can promote the growth of NSCLC cells by targeting miR-539.
探讨长链非编码核糖核酸 00460（LINC00460）对非小细胞肺癌（NSCLC）细胞增殖和凋亡的影响。
本研究利用基因表达谱交互分析（GEPIA）网站和癌症基因组图谱（TCGA）数据库分析了 LINC00460 在肺癌组织中的表达及其在 NSCLC 患者中的预后潜力。然后，通过噻唑蓝（MTT）比色法、集落形成实验和流式细胞术观察沉默 LINC00460 对 A549 细胞增殖和凋亡的影响。此外，还进行了双荧光素酶报告基因检测和逆转录-定量聚合酶链反应（RT-qPCR）检测 LINC00460 与 microRNA（miR）-539 之间的靶向关系。采用 MTT 比色法检测 LINC00460/miR-539 轴在 A549 细胞增殖中的生物学作用。
结果表明，LINC00460 在 NSCLC 组织和细胞系中的表达水平明显上调，且与总生存期（OS）呈负相关。根据体外实验结果，沉默 LINC00460 可抑制 A549 细胞的增殖，但促进其凋亡。双荧光素酶报告基因检测结果显示，miR-539 可被 LINC00460 直接靶向，并参与 LINC00460 介导的 A549 细胞增殖。
LINC00460 在 NSCLC 组织中高表达，可通过靶向 miR-539 促进 NSCLC 细胞的生长。
