Department of Otolaryngology Head and Neck Surgery, Second Affiliated Hospital of Xi'an Jiaotong University, Xian, China.
Eur Rev Med Pharmacol Sci. 2020 Jun;24(12):6786-6793. doi: 10.26355/eurrev_202006_21667.
This study aims to investigate the potential regulatory effect of forkhead box M1 (FOXM1) on laryngeal carcinoma (LC) and the underlying mechanisms.
Tumor tissues were obtained from 80 patients diagnosed with LC in our hospital. Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) and Western blot analysis were used to detect the expression levels of FOXM1 in LC tissues and cell lines. Transfection of small interfering RNA (si-RNA) was conducted to knockdown the expression level of FOXM1. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay and cell colony assay were conducted to measure the changes in cell proliferation capacity influenced by FOXM1. Finally, invasion and migration ability was evaluated by the transwell assay.
FOXM1 was found upregulated in LC tissues and cells. Transfection of FOXM1 siRNA in LC cells successfully inhibited the expression of FOXM1. The knockdown of FOXM1 resulted in reduced proliferation, invasion, and migration of LC cells. Further studies indicated that the knockdown of FOXM1 suppressed the ratio of p-AKT/AKT. Besides, the impaired proliferation, invasion, and migration of LC cells induced by FOXM1 knockdown could be counteracted by application of the AKT activator Sc79.
The present work demonstrated that the knockdown of FOXM1 suppressed the proliferation, invasion, and migration of LC cells by the AKT signaling pathway.
本研究旨在探讨叉头框蛋白 M1（FOXM1）对喉癌（LC）的潜在调控作用及其机制。
收集我院 80 例 LC 患者的肿瘤组织，采用定量实时聚合酶链反应（qRT-PCR）和 Western blot 分析检测 LC 组织及细胞系中 FOXM1 的表达水平。通过转染小干扰 RNA（si-RNA）降低 FOXM1 的表达水平。采用 MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）法和细胞集落形成实验检测 FOXM1 对细胞增殖能力的影响。最后，通过 Transwell 实验评估细胞的侵袭和迁移能力。
FOXM1 在 LC 组织和细胞中呈高表达。在 LC 细胞中转染 FOXM1 siRNA 可成功抑制 FOXM1 的表达。FOXM1 的下调导致 LC 细胞的增殖、侵袭和迁移能力降低。进一步研究表明，FOXM1 的下调抑制了 p-AKT/AKT 比值。此外，FOXM1 敲低诱导的 LC 细胞增殖、侵袭和迁移受损可被 AKT 激活剂 Sc79 逆转。
本研究表明，FOXM1 的下调通过 AKT 信号通路抑制 LC 细胞的增殖、侵袭和迁移。
