Otsuka T, Okamura S, Ohhara N, Harada M, Hayashi S, Yamaga S, Omori F, Niho Y
First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Int J Immunopharmacol. 1988;10(5):587-91. doi: 10.1016/0192-0561(88)90077-x.
Bestatin, (2S, 3R)-3-amino-2-hydroxy-4-phenylbutyryl-L-leucine, is a small molecular immunomodifier. Effects of this compound on human immune function were studied, in vitro, using the human B-cell colony formation technique. B-cell colonies were obtained from enriched B-cell populations placed in conditioned methylcellulose medium containing stimulators and irradiated T-cells as feeders. Addition to the culture of Bestatin at concentrations of 0.1 microgram/ml and 1 microgram/ml led to a significant increase (P less than 0.05) in the number of B-cell colonies and this effect was abolished when irradiated T-cells were not added to the culture. Bestatin increased soluble factor production induced by phytohemagglutinin (PHA)-stimulated T-cells. Such findings suggest that T-cells probably mediate this stimulatory effect of Bestatin on B-cell colony formation.
贝司他汀，即(2S, 3R)-3-氨基-2-羟基-4-苯基丁酰-L-亮氨酸，是一种小分子免疫调节剂。利用人B细胞集落形成技术，在体外研究了该化合物对人体免疫功能的影响。B细胞集落是从富集的B细胞群体中获得的，这些细胞被置于含有刺激物和经辐照的T细胞作为饲养细胞的条件甲基纤维素培养基中。以0.1微克/毫升和1微克/毫升的浓度向培养物中添加贝司他汀，导致B细胞集落数量显著增加（P<0.05），当不向培养物中添加经辐照的T细胞时，这种效应消失。贝司他汀增加了植物血凝素（PHA）刺激的T细胞诱导的可溶性因子的产生。这些发现表明，T细胞可能介导了贝司他汀对B细胞集落形成的这种刺激作用。
