Lindholm D, Heumann R, Hengerer B, Thoenen H
Department of Neurochemistry, Max-Planck-Institute for Psychiatry, Martinsried/Munich, Federal Republic of Germany.
J Biol Chem. 1988 Nov 5;263(31):16348-51.
The lymphokine interleukin 1 (IL-1) rapidly increases the levels of mRNA encoding nerve growth factor (NGF) in primary cultures of fibroblasts isolated from adult rat sciatic nerves. The effect of IL-1 on NGF-mRNA was not inhibited by cycloheximide, which itself increased the NGF-mRNA. From the blocking ability of actinomycin D, it was deduced that the IL-1 action depends on continuous RNA synthesis and that the half-life of NGF-mRNA is about 90 min. Nuclear run-on studies showed that, although IL-1 enhances the low basic NGF-mRNA transcription, the main effect of IL-1 results from stabilization of the NGF-mRNA. In addition, indirect evidence is presented indicating that the IL-1 effect of NGF-mRNA levels involves activation of phospholipase A2.
淋巴因子白细胞介素1（IL-1）可迅速提高从成年大鼠坐骨神经分离出的成纤维细胞原代培养物中编码神经生长因子（NGF）的mRNA水平。IL-1对NGF-mRNA的作用不受放线菌酮的抑制，而放线菌酮本身会增加NGF-mRNA的水平。从放线菌素D的阻断能力推断，IL-1的作用依赖于持续的RNA合成，且NGF-mRNA的半衰期约为90分钟。细胞核连续转录研究表明，尽管IL-1增强了低水平基础NGF-mRNA的转录，但IL-1的主要作用源于NGF-mRNA的稳定性。此外，有间接证据表明，IL-1对NGF-mRNA水平的影响涉及磷脂酶A2的激活。
