School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.
School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, China.
Biomed Chromatogr. 2020 Nov;34(11):e4940. doi: 10.1002/bmc.4940. Epub 2020 Jul 22.
Shaoyao Gancao decoction (SGD) is a famous Chinese traditional prescription for treating liver injury. In this research, we investigated the therapeutic effects of SGD on liver injury and its metabolic mechanisms using H NMR and UPLC-MS. Serum biochemical indicators and histopathological methods were used to determine the mechanism of action of SGD in treating liver injury. An orthogonal partial least squares discriminant analysis method was used to screen potential metabolic markers, and the MetaboAnalyst and KEGG PATHWAY databases were used to find relevant metabolic pathways. A total of 26 significant metabolites were identified with significant changes in their abundance levels, and these metabolites are involved in many metabolic pathways such as amino acid and lipid metabolism. The changes in biomarker levels reveal the therapeutic effect of SGD on liver injury, which is of great significance to speculate on possible metabolic mechanisms.
芍药甘草汤（SGD）是一种著名的治疗肝损伤的中药方剂。本研究采用 1H NMR 和 UPLC-MS 技术，探讨了 SGD 治疗肝损伤的疗效及其代谢机制。采用血清生化指标和组织病理学方法确定了 SGD 治疗肝损伤的作用机制。采用正交偏最小二乘判别分析方法筛选潜在的代谢标志物，并采用 MetaboAnalyst 和 KEGG PATHWAY 数据库寻找相关代谢途径。共鉴定出 26 个有显著变化的显著代谢物，这些代谢物涉及氨基酸和脂质代谢等多种代谢途径。生物标志物水平的变化揭示了 SGD 治疗肝损伤的疗效，这对推测可能的代谢机制具有重要意义。
