Punjabi C J, Wood D D, Wooley P H
Department of Immunology, Ayerst Laboratories Research, Princeton, NJ 08543-8000.
J Immunol. 1988 Dec 1;141(11):3819-22.
An IgG2a hybridoma antibody (BC-10) was obtained by a myeloma fusion with lymphocytes from B10.RIII mice immunized against native bovine type II collagen. This anti-collagen monoclonal exhibited extensive cross-reactivity with several type II collagen species. BC-10 was found to have self-associating properties, but not the specificity of a typical IgG rheumatoid factor, inasmuch as this mAb bound to F(ab')2 fragments of itself and of normal mouse IgG. Self binding was inhibited by the association of BC-10 with type II collagen, and inhibition assays indicated that antibodies with the capacity to inhibit BC-10 binding to collagen were present in the sera from B10.RIII arthritic mice, but not from DBA/1 LacJ arthritic mice. Joint inflammation and histopathologic features consistent with arthritis were observed in mice injected with the BC-10 hybridoma.
通过将骨髓瘤与来自用天然牛II型胶原免疫的B10.RIII小鼠的淋巴细胞进行融合，获得了一种IgG2a杂交瘤抗体（BC - 10）。这种抗胶原单克隆抗体与几种II型胶原种类表现出广泛的交叉反应性。发现BC - 10具有自缔合特性，但不具有典型IgG类风湿因子的特异性，因为这种单克隆抗体能结合自身以及正常小鼠IgG的F(ab')2片段。BC - 10与II型胶原的缔合可抑制自身结合，抑制试验表明，B10.RIII关节炎小鼠血清中存在能够抑制BC - 10与胶原结合的抗体，而DBA/1 LacJ关节炎小鼠血清中则不存在。在用BC - 10杂交瘤注射的小鼠中观察到了与关节炎一致的关节炎症和组织病理学特征。
