Tentori L, Longo D L, Zuñiga-Pflucker J C, Wing C, Kruisbeek A M
Division of Cancer Treatment, National Cancer Institute, Frederick, Maryland 21701.
J Exp Med. 1988 Nov 1;168(5):1741-7. doi: 10.1084/jem.168.5.1741.
The role of the IL-2-IL-2-R pathway in thymocyte differentiation in vivo is unknown. We have examined fetal thymocyte development in vivo, under conditions where all IL-2-R were saturated from day 13 of gestation with anti-IL-2-R mAbs that were previously shown to render mature T cells unable to respond to IL-2. This produced a dramatic change in the composition of developing T cells: thymocytes from day 1 neonatal mice born to anti-IL-2-R-treated mothers did not contain CD4+ or CD8+ single-positive cell populations. In addition, no generation of surface TCR beta chain-expressing T cells or antigen-reactive functional T cells occurred in treated mice. These data suggest that IL-2-IL-2-R interactions provide signals crucial to in vivo intrathymic development of mature T cells.
白细胞介素-2（IL-2）-白细胞介素-2受体（IL-2-R）通路在体内胸腺细胞分化中的作用尚不清楚。我们在妊娠第13天起所有IL-2-R均被抗IL-2-R单克隆抗体饱和的条件下，研究了体内胎儿胸腺细胞的发育情况，此前已证明这些抗体可使成熟T细胞无法对IL-2作出反应。这导致发育中的T细胞组成发生了显著变化：接受抗IL-2-R治疗的母亲所生的1日龄新生小鼠的胸腺细胞中不包含CD4+或CD8+单阳性细胞群体。此外，在接受治疗的小鼠中未出现表达表面TCRβ链的T细胞或抗原反应性功能性T细胞。这些数据表明，IL-2-IL-2-R相互作用为成熟T细胞在体内胸腺内的发育提供了关键信号。
