Center for drug screening and research, School of Geography and Environmental Engineering, Gannan Normal University, Ganzhou, 341000, Jiangxi, China; College of life and science, Gannan Normal University, Ganzhou, 341000, Jiangxi, China.
Jiangxi Engineering Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases, Ji'an, 343009, Jiangxi, China; Jiangxi Key Laboratory of Developmental Biology of Organs, Jinggangshan University, Ji'an, 343009, Jiangxi, China.
Chemosphere. 2020 Nov;259:127380. doi: 10.1016/j.chemosphere.2020.127380. Epub 2020 Jun 12.
Fomesafen is widely used in agriculture and can be detected in the environment and agricultural products. Research on the developmental toxicity of fomesafen in animals is currently very limited. Here, we used zebrafish as an animal model to evaluate the toxicity of fomesafen in developing aquatic vertebrates and higher animals. From 6h to 72h following fertilization, exposure of zebrafish embryos to 5, 10 and 20 mg/L of fomesafen resulted in pericardial edema, a reduction in heart rate, shortening of body length, and yolk sac edema. Fomesafen reduced the number of immune cells such as neutrophils and macrophages, increased the expression of a number of inflammatory factors, induced the up-regulation of the oxidative stress response and apoptosis, and disrupted the activity of enzymes related to nerve development, which affected the motility of the embryos. In conclusion, the results provide new evidence for the comprehensive assessment of fomesafen toxicity in aquatic vertebrates.
氟磺胺草醚在农业中被广泛使用，并可能存在于环境和农产品中。目前，关于氟磺胺草醚对动物发育毒性的研究非常有限。在这里，我们使用斑马鱼作为动物模型，来评估氟磺胺草醚对水生脊椎动物和高等动物发育的毒性。从受精后 6 小时到 72 小时，斑马鱼胚胎暴露于 5、10 和 20 mg/L 的氟磺胺草醚中会导致心包水肿、心率降低、体长缩短和卵黄囊水肿。氟磺胺草醚减少了中性粒细胞和巨噬细胞等免疫细胞的数量，增加了许多炎症因子的表达，诱导了氧化应激反应和细胞凋亡的上调，并破坏了与神经发育相关的酶的活性，从而影响了胚胎的运动能力。总之，这些结果为全面评估氟磺胺草醚对水生脊椎动物的毒性提供了新的证据。
