Department of Biochemistry, BioFrontiers Institute, University of Colorado Boulder, 3415 Colorado Avenue, Boulder, CO 80309, USA.
Department of Biochemistry, BioFrontiers Institute, University of Colorado Boulder, 3415 Colorado Avenue, Boulder, CO 80309, USA.
Cell Chem Biol. 2020 Aug 20;27(8):891-903. doi: 10.1016/j.chembiol.2020.06.010. Epub 2020 Jul 7.
The central dogma teaches us that DNA makes RNA, which in turn makes proteins, the main building blocks of the cell. But this over simplified linear transmission of information overlooks the vast majority of the genome produces RNAs that do not encode proteins and the myriad ways that RNA regulates cellular functions. Historically, one of the challenges in illuminating RNA biology has been the lack of tools for visualizing RNA in live cells. But clever approaches for exploiting RNA binding proteins, in vitro RNA evolution, and chemical biology have resulted in significant advances in RNA visualization tools in recent years. This review provides an overview of current tools for tagging RNA with fluorescent probes and tracking their dynamics, localization andfunction in live mammalian cells.
中心法则告诉我们，DNA 生成 RNA，RNA 进而生成蛋白质，蛋白质是细胞的主要结构单元。但这种过于简化的线性信息传递忽略了绝大多数基因组产生的非编码蛋白质的 RNA，以及 RNA 调节细胞功能的多种方式。从历史上看，阐明 RNA 生物学的挑战之一一直是缺乏在活细胞中可视化 RNA 的工具。但是，近年来，利用 RNA 结合蛋白、体外 RNA 进化和化学生物学的巧妙方法已经在 RNA 可视化工具方面取得了重大进展。这篇综述概述了目前用于用荧光探针标记 RNA 并追踪其在活哺乳动物细胞中的动态、定位和功能的工具。
