使用 RNA 稳定的荧光蛋白对 mRNA 进行实时成像。
Live imaging of mRNA using RNA-stabilized fluorogenic proteins.
机构信息
Department of Pharmacology, Weill Cornell Medicine, Cornell University, New York, NY, USA.
Oncode Institute, Hubrecht Institute, KNAW and University Medical Center Utrecht, Utrecht, The Netherlands.
出版信息
Nat Methods. 2019 Sep;16(9):862-865. doi: 10.1038/s41592-019-0531-7. Epub 2019 Aug 30.
Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. In this communication, fluorogenic proteins whose fluorescence is activated by RNA aptamers are described. Fluorogenic proteins are highly unstable until they bind RNA aptamers inserted into messenger RNAs, resulting in fluorescent RNA-protein complexes that enable live imaging of mRNA in living cells.
荧光 RNA 适体与原本非荧光的染料结合并激活其荧光。然而，由于适合用于活细胞的荧光染料数量有限，因此荧光适体受到限制。在本通讯中，描述了由 RNA 适体激活荧光的荧光蛋白。荧光蛋白在结合插入信使 RNA 中的 RNA 适体之前极不稳定，导致形成荧光 RNA-蛋白复合物，从而能够对活细胞中的 mRNA 进行实时成像。
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