Department of Pharmacy and Pharmaceutical Engineering, School of Biotechnology and Health Sciences, Wuyi University , Jiangmen Province, Guangdong, 529020, China.
Department of Otolaryngology, Guangdong Provincial People's Hospital , Guangzhou, China.
Biosci Biotechnol Biochem. 2020 Oct;84(10):2069-2076. doi: 10.1080/09168451.2020.1789837. Epub 2020 Jul 8.
12-O-tetradecanoylphorbol-13-acetate (TPA), is a major active constituent of the seed oil of L., has pharmacological activity for the treatment of acute myeloid leukemia patients. Diethyldithiocarbamate (DTC) is a potent inhibitor of NF-κB show activity of anticancer. In this study, we determined the effect of DTC and TPA in combination on HL-60 cells cultured and . In this study, we have shown that DTC and TPA synergistically inhibited the growth of HL-60 cells and strongly induced apoptosis in the cells. Mechanistic studies showed that the combined effects of DTC and TPA were associated with a decrease in Bcl-2. The animal experiment showed that the combination of DTC and TPA more potently inhibited the growth of HL-60 tumors than either agent alone. Our results indicate that the administration of TPA and DTC in combination may be an effective strategy for inhibiting the growth of acute myeloid leukemia cells.
12-O-十四烷酰佛波醇-13-乙酸酯（TPA）是 L. 种子油中的主要活性成分，具有治疗急性髓系白血病患者的药理学活性。二乙基二硫代氨基甲酸盐（DTC）是一种有效的 NF-κB 抑制剂，具有抗癌活性。在这项研究中，我们确定了 DTC 和 TPA 联合对培养的 HL-60 细胞的影响。在这项研究中，我们已经表明，DTC 和 TPA 协同抑制 HL-60 细胞的生长，并强烈诱导细胞凋亡。机制研究表明，DTC 和 TPA 的联合作用与 Bcl-2 的减少有关。动物实验表明，DTC 和 TPA 的联合给药比单独使用任何一种药物更能有效地抑制 HL-60 肿瘤的生长。我们的结果表明，联合使用 TPA 和 DTC 可能是抑制急性髓系白血病细胞生长的有效策略。
