• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

KLF5 通过经典的 Wnt/β-catenin 信号通路调节鸡骨骼肌萎缩。

KLF5 regulates chicken skeletal muscle atrophy via the canonical Wnt/β-catenin signaling pathway.

机构信息

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, 211 Huiming Road, Wenjiang, Sichuan province, Chengdu 611130, China.

Animal Breeding and Genetics Key Laboratory of Sichuan Province, 7 Niusha Road, Jinjiang, Sichuan province, Chengdu 610066, China.

出版信息

Exp Anim. 2020 Nov 12;69(4):430-440. doi: 10.1538/expanim.20-0046. Epub 2020 Jul 7.

DOI:10.1538/expanim.20-0046
PMID:32641593
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7677084/
Abstract

Recent studies in mice suggested that KLF5 (Kruppel like factor 5), a zinc-finger transcription factor, plays an important role in skeletal muscle development and regeneration. As an important factor in the process of muscle development, KLF5 participates in the regulation of the cell cycle, cell survival, and cell dryness under different environmental conditions, but it is not clear whether KLF5 participates in muscle atrophy. Therefore, we investigated whether KLF5 can regulate the atrophy of chicken satellite cells in vitro and examined its mechanism of action. qPCR showed that KLF5 gene knockdown promoted the expression of key genes in muscle atrophy. Subsequently, we sequenced and analyzed the transcriptomes of KLF5 silenced and control cells, and we showed that the differentially expressed genes were mainly enriched in 10 signaling pathways (P<0.05), with differential gene and enrichment analyses indicating that the Wnt signaling pathways are extremely important. In conclusion, our results indicate that KLF5 may regulate the atrophy of chicken skeletal muscle through the Wnt/β-catenin signaling pathway.

摘要

最近在小鼠中的研究表明，KLF5（Kruppel 样因子 5），一种锌指转录因子，在骨骼肌发育和再生中发挥重要作用。作为肌肉发育过程中的一个重要因素，KLF5 参与调节细胞周期、细胞存活和不同环境条件下的细胞干性，但尚不清楚 KLF5 是否参与肌肉萎缩。因此，我们研究了 KLF5 是否可以调节体外鸡卫星细胞的萎缩，并研究了其作用机制。qPCR 显示，KLF5 基因敲低促进了肌肉萎缩关键基因的表达。随后，我们对 KLF5 沉默和对照细胞的转录组进行了测序和分析，结果表明差异表达基因主要富集在 10 个信号通路中（P<0.05），差异基因和富集分析表明 Wnt 信号通路非常重要。总之，我们的结果表明，KLF5 可能通过 Wnt/β-catenin 信号通路调节鸡骨骼肌的萎缩。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/eb99e540534c/expanim-69-430-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/07d2a90448a7/expanim-69-430-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/efa99390abeb/expanim-69-430-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/5defd3310630/expanim-69-430-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/9deffb54644d/expanim-69-430-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/1d52bfa15f5e/expanim-69-430-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/a3c356daf8fe/expanim-69-430-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/925d64e1c94c/expanim-69-430-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/d988b481c056/expanim-69-430-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/e58d11be3bae/expanim-69-430-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/eb99e540534c/expanim-69-430-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/07d2a90448a7/expanim-69-430-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/efa99390abeb/expanim-69-430-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/5defd3310630/expanim-69-430-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/9deffb54644d/expanim-69-430-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/1d52bfa15f5e/expanim-69-430-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/a3c356daf8fe/expanim-69-430-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/925d64e1c94c/expanim-69-430-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/d988b481c056/expanim-69-430-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/e58d11be3bae/expanim-69-430-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5e8/7677084/eb99e540534c/expanim-69-430-g010.jpg

相似文献

1
KLF5 regulates chicken skeletal muscle atrophy via the canonical Wnt/β-catenin signaling pathway.KLF5 通过经典的 Wnt/β-catenin 信号通路调节鸡骨骼肌萎缩。
Exp Anim. 2020 Nov 12;69(4):430-440. doi: 10.1538/expanim.20-0046. Epub 2020 Jul 7.
2
Coordinated action of Axin1 and Axin2 suppresses β-catenin to regulate muscle stem cell function.Axin1和Axin2的协同作用抑制β-连环蛋白以调节肌肉干细胞功能。
Cell Signal. 2015 Aug;27(8):1652-65. doi: 10.1016/j.cellsig.2015.03.025. Epub 2015 Apr 10.
3
The Wnt effector transcription factor 7-like 2 positively regulates oligodendrocyte differentiation in a manner independent of Wnt/β-catenin signaling.Wnt效应转录因子7样2以独立于Wnt/β-连环蛋白信号传导的方式正向调节少突胶质细胞分化。
J Neurosci. 2015 Mar 25;35(12):5007-22. doi: 10.1523/JNEUROSCI.4787-14.2015.
4
MicroRNA-140 inhibits skeletal muscle glycolysis and atrophy in endotoxin-induced sepsis in mice via the WNT signaling pathway.MicroRNA-140 通过 WNT 信号通路抑制内毒素诱导的脓毒症小鼠骨骼肌糖酵解和萎缩。
Am J Physiol Cell Physiol. 2019 Aug 1;317(2):C189-C199. doi: 10.1152/ajpcell.00419.2018. Epub 2019 May 1.
5
Resistance training prevents muscle fibrosis and atrophy via down-regulation of C1q-induced Wnt signaling in senescent mice.抗阻训练通过下调衰老小鼠 C1q 诱导的 Wnt 信号通路预防肌肉纤维化和萎缩。
FASEB J. 2018 Jul;32(7):3547-3559. doi: 10.1096/fj.201700772RRR. Epub 2018 Jan 30.
6
Klf5 regulates muscle differentiation by directly targeting muscle-specific genes in cooperation with MyoD in mice.在小鼠中，Klf5通过与MyoD协同直接靶向肌肉特异性基因来调节肌肉分化。
Elife. 2016 Oct 15;5:e17462. doi: 10.7554/eLife.17462.
7
R-spondin 2 promotes osteoblastic differentiation of immature human periodontal ligament cells through the Wnt/β-catenin signaling pathway.R 型分泌蛋白 2 通过 Wnt/β-连环蛋白信号通路促进未成熟人牙周膜细胞的成骨细胞分化。
J Periodontal Res. 2019 Apr;54(2):143-153. doi: 10.1111/jre.12611. Epub 2018 Oct 4.
8
Wnt/beta-catenin signaling activates growth-control genes during overload-induced skeletal muscle hypertrophy.Wnt/β-连环蛋白信号通路在超负荷诱导的骨骼肌肥大过程中激活生长控制基因。
Am J Physiol Cell Physiol. 2005 Oct;289(4):C853-9. doi: 10.1152/ajpcell.00093.2005. Epub 2005 May 11.
9
Canonical Wnt signaling regulates Foxc1/2 expression in P19 cells.经典 Wnt 信号通路调控 P19 细胞中 Foxc1/2 的表达。
Differentiation. 2010 Jan;79(1):31-40. doi: 10.1016/j.diff.2009.08.008. Epub 2009 Sep 25.
10
Ectonucleotide pyrophosphatase 2 (ENPP2) plays a crucial role in myogenic differentiation through the regulation by WNT/β-Catenin signaling.核苷酸焦磷酸酶 2（ENPP2）通过 WNT/β-连环蛋白信号通路的调节在成肌分化中发挥关键作用。
Int J Biochem Cell Biol. 2020 Jan;118:105661. doi: 10.1016/j.biocel.2019.105661. Epub 2019 Dec 2.

引用本文的文献

1
Targeting cancer-induced skeletal damage: a holistic approach to understanding pathophysiology, mechanisms, and management solutions.靶向癌症引起的骨骼损伤：一种全面理解病理生理学、机制及管理解决方案的方法。
Am J Cancer Res. 2025 Apr 15;15(4):1494-1516. doi: 10.62347/QFHJ2430. eCollection 2025.
2
Integration of whole genome resequencing and transcriptome sequencing to identify candidate genes for tall and short traits in Baicheng Fatty chickens.整合全基因组重测序和转录组测序以鉴定白城脂肪鸡高矮性状的候选基因。
Front Vet Sci. 2025 Feb 27;12:1534742. doi: 10.3389/fvets.2025.1534742. eCollection 2025.
3
Genome-Wide Identification, Evolution, and miRNA-22 Regulation of () Gene Family in Chicken ().

本文引用的文献

1
functions in proliferation, differentiation, and apoptosis of chicken satellite cells.在鸡卫星细胞的增殖、分化和凋亡中发挥作用。
3 Biotech. 2019 Jun;9(6):222. doi: 10.1007/s13205-019-1752-2. Epub 2019 May 20.
2
Klf5 regulates muscle differentiation by directly targeting muscle-specific genes in cooperation with MyoD in mice.在小鼠中，Klf5通过与MyoD协同直接靶向肌肉特异性基因来调节肌肉分化。
Elife. 2016 Oct 15;5:e17462. doi: 10.7554/eLife.17462.
3
The effect of temperature on proliferation and differentiation of chicken skeletal muscle satellite cells isolated from different muscle types.
鸡（）中（）基因家族的全基因组鉴定、进化及miRNA-22调控
Animals (Basel). 2024 Sep 6;14(17):2594. doi: 10.3390/ani14172594.
4
Integrative ATAC-seq and RNA-seq Analysis of the Longissimus Dorsi Muscle of Gannan Yak and Jeryak.甘南高寒牦牛和吉仁牦牛背最长肌的整合 ATAC-seq 和 RNA-seq 分析
Int J Mol Sci. 2024 May 30;25(11):6029. doi: 10.3390/ijms25116029.
5
Disease-driven top predator decline affects mesopredator population genomic structure.疾病导致的顶级捕食者数量下降影响了中型捕食者种群的基因组结构。
Nat Ecol Evol. 2024 Feb;8(2):293-303. doi: 10.1038/s41559-023-02265-9. Epub 2024 Jan 8.
6
Potential application of anti-osteoporotic therapy to relieve sarcopenia in the elderly.抗骨质疏松治疗在缓解老年人肌肉减少症方面的潜在应用。
Ann Med Surg (Lond). 2023 Oct 4;85(12):6008-6012. doi: 10.1097/MS9.0000000000001352. eCollection 2023 Dec.
7
The Integration of Genome-Wide DNA Methylation and Transcriptomics Identifies the Potential Genes That Regulate the Development of Skeletal Muscles in Ducks.全基因组 DNA 甲基化和转录组学的整合鉴定了调控鸭骨骼肌发育的潜在基因。
Int J Mol Sci. 2023 Oct 23;24(20):15476. doi: 10.3390/ijms242015476.
8
Genome-Wide Analysis of the Family in Chicken: Characterization and Expression Profile.鸡中该家族的全基因组分析：特征与表达谱
Animals (Basel). 2023 Apr 22;13(9):1429. doi: 10.3390/ani13091429.
9
Sarcopenia: Molecular regulatory network for loss of muscle mass and function.肌肉减少症：肌肉质量和功能丧失的分子调控网络。
Front Nutr. 2023 Feb 2;10:1037200. doi: 10.3389/fnut.2023.1037200. eCollection 2023.
10
Adenosine monophosphate activated protein kinase contributes to skeletal muscle health through the control of mitochondrial function.单磷酸腺苷激活蛋白激酶通过控制线粒体功能来促进骨骼肌健康。
Front Pharmacol. 2022 Oct 20;13:947387. doi: 10.3389/fphar.2022.947387. eCollection 2022.
温度对从不同肌肉类型分离的鸡骨骼肌卫星细胞增殖和分化的影响。
Physiol Rep. 2016 Apr;4(8). doi: 10.14814/phy2.12770. Epub 2016 Apr 27.
4
Coordinated action of Axin1 and Axin2 suppresses β-catenin to regulate muscle stem cell function.Axin1和Axin2的协同作用抑制β-连环蛋白以调节肌肉干细胞功能。
Cell Signal. 2015 Aug;27(8):1652-65. doi: 10.1016/j.cellsig.2015.03.025. Epub 2015 Apr 10.
5
Transiently active Wnt/β-catenin signaling is not required but must be silenced for stem cell function during muscle regeneration.短暂激活的 Wnt/β-catenin 信号传导对于肌肉再生过程中的干细胞功能不是必需的，但必须被沉默。
Stem Cell Reports. 2014 Sep 9;3(3):475-88. doi: 10.1016/j.stemcr.2014.06.019. Epub 2014 Jul 31.
6
Wnt7a stimulates myogenic stem cell motility and engraftment resulting in improved muscle strength.Wnt7a 可刺激成肌干细胞的迁移，从而提高其植入效率，增强肌肉力量。
J Cell Biol. 2014 Apr 14;205(1):97-111. doi: 10.1083/jcb.201310035. Epub 2014 Apr 7.
7
Mechanisms regulating skeletal muscle growth and atrophy.调节骨骼肌生长和萎缩的机制。
FEBS J. 2013 Sep;280(17):4294-314. doi: 10.1111/febs.12253. Epub 2013 Apr 17.
8
Cellular and molecular mechanisms of muscle atrophy.肌肉萎缩的细胞和分子机制。
Dis Model Mech. 2013 Jan;6(1):25-39. doi: 10.1242/dmm.010389.
9
Wnt7a treatment ameliorates muscular dystrophy.Wnt7a 治疗可改善肌肉萎缩症。
Proc Natl Acad Sci U S A. 2012 Dec 11;109(50):20614-9. doi: 10.1073/pnas.1215765109. Epub 2012 Nov 26.
10
Atrogin-1, MuRF-1, and sarcopenia.肌萎缩蛋白-1（Atrogin-1）、泛素连接酶 E3 家族成员 1（MuRF-1）与肌肉减少症。
Endocrine. 2013 Feb;43(1):12-21. doi: 10.1007/s12020-012-9751-7. Epub 2012 Jul 20.