Department of Plant Sciences, Laboratory of Molecular Biology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
Department of Terrestrial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Droevendaalsesteeg 10, 6708 PB, Wageningen, The Netherlands.
ISME J. 2020 Oct;14(10):2433-2448. doi: 10.1038/s41396-020-0695-2. Epub 2020 Jul 8.
As a model for genetic studies, Arabidopsis thaliana (Arabidopsis) offers great potential to unravel plant genome-related mechanisms that shape the root microbiome. However, the fugitive life history of this species might have evolved at the expense of investing in capacity to steer an extensive rhizosphere effect. To determine whether the rhizosphere effect of Arabidopsis is different from other plant species that have a less fugitive life history, we compared the root microbiome of Arabidopsis to eight other, later succession plant species from the same habitat. The study included molecular analysis of soil, rhizosphere, and endorhizosphere microbiome both from the field and from a laboratory experiment. Molecular analysis revealed that the rhizosphere effect (as quantified by the number of enriched and depleted bacterial taxa) was ~35% lower than the average of the other eight species. Nevertheless, there are numerous microbial taxa differentially abundant between soil and rhizosphere, and they represent for a large part the rhizosphere effects of the other plants. In the case of fungal taxa, the number of differentially abundant taxa in the Arabidopsis rhizosphere is 10% of the other species' average. In the plant endorhizosphere, which is generally more selective, the rhizosphere effect of Arabidopsis is comparable to other species, both for bacterial and fungal taxa. Taken together, our data imply that the rhizosphere effect of the Arabidopsis is smaller in the rhizosphere, but equal in the endorhizosphere when compared to plant species with a less fugitive life history.
作为遗传研究的模型，拟南芥（Arabidopsis）具有很大的潜力来揭示与植物基因组相关的机制，这些机制塑造了根际微生物组。然而，这种物种的逃逸生活史可能是以牺牲投资能力为代价的，这种能力旨在引导广泛的根际效应。为了确定拟南芥的根际效应是否与具有较少逃逸生活史的其他植物物种不同，我们将拟南芥的根际微生物组与来自同一栖息地的其他八个后续植物物种进行了比较。该研究包括对土壤、根际和根内微生物组的分子分析，这些分析来自野外和实验室实验。分子分析表明，根际效应（通过丰富和耗尽细菌分类群的数量来量化）比其他八个物种的平均值低约 35%。然而，土壤和根际之间有许多微生物类群丰度存在差异，它们在很大程度上代表了其他植物的根际效应。在真菌类群的情况下，拟南芥根际中丰度差异较大的分类群数量占其他物种平均数量的 10%。在通常更具选择性的植物根内区，拟南芥的根际效应与具有较少逃逸生活史的植物物种的根际效应相当，无论是细菌还是真菌类群。总之，我们的数据表明，与具有较少逃逸生活史的植物物种相比，拟南芥的根际效应在根际较小，但在内根区相等。
