Department of Obstetrics, Binzhou Central Hospital, Binzhou, Shandong, China.
Department of Emergency, Binzhou Central Hospital, Binzhou, Shandong, China.
Kaohsiung J Med Sci. 2020 Nov;36(11):878-884. doi: 10.1002/kjm2.12273. Epub 2020 Jul 9.
It has been reported microRNA-301b (miR-301b) was involved in the tumorigenesis of some cancers, but it has not been investigated in cervical carcinoma yet. In this study, miR-301b was found significantly upregulated in cervical carcinoma, and patients with high miR-301b expression had a shorter overall survival. When miR-301b was knocked down in cervical carcinoma cells, the cell growth could be significantly abolished. Our further studies showed miR-301b targeted RNF38, and inhibited its expression in cervical carcinoma cells. Moreover, RNF38 was found downregulated in cervical carcinoma, and miR-301b expression in cervical tissues was found negatively correlated with RNF38 expression. In addition, overexpression of RNF38 significantly inhibited cervical carcinoma cell growth, but overexpression of miR-301b suppressed RNF38-induced cell growth inhibition in cervical carcinoma. Collectively, this study suggested miR-301b could be a novel target for cervical carcinoma treatment.
已有报道称 microRNA-301b（miR-301b）参与了一些癌症的肿瘤发生，但尚未在宫颈癌中进行研究。在本研究中，发现 miR-301b 在宫颈癌中显著上调，miR-301b 高表达的患者总生存期较短。当在宫颈癌细胞中敲低 miR-301b 时，细胞生长可显著被抑制。我们的进一步研究表明 miR-301b 靶向 RNF38，并抑制其在宫颈癌细胞中的表达。此外，在宫颈癌中发现 RNF38 下调，并且在宫颈癌组织中发现 miR-301b 的表达与 RNF38 的表达呈负相关。此外，过表达 RNF38 显著抑制宫颈癌细胞生长，但过表达 miR-301b 抑制了 RNF38 诱导的宫颈癌细胞生长抑制。总之，本研究表明 miR-301b 可能成为宫颈癌治疗的新靶点。
